High-salt diet advances molecular circadian rhythms in mouse peripheral tissues
High-salt diet advances molecular circadian rhythms in mouse peripheral tissues
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DOI：
10.1016/j.bbrc.2010.09.072
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发表时间：
2010-11-05
影响因子：
3.1
通讯作者：
Kobori, Masuko
中科院分区：
文献类型：
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作者：
Oike, Hideaki;Nagai, Kanji;Kobori, Masuko
膳食化合物影响多种基因的表达，并在改变生理和代谢状态方面发挥重要作用。然而，人们对食物成分在调节昼夜节律基因表达中的作用知之甚少。在这里，我们发现，随意喂食高盐(HS)超过2周的小鼠，肝脏、肾脏和肺中时钟基因的表达时间提前了约3h，但没有改变昼夜摄食、饮水和运动的节律。聚焦DNA芯片分析显示，肝脏中与代谢相关的许多基因的表达阶段也提前了。在外周组织阶段推进之前，负责葡萄糖吸收的钠-葡萄糖协同转运体1(SGLT1)和葡萄糖转运体2(Glut2)的mRNA在空肠组织中的表达增加。此外，食用HS饮食后，血糖摄取量比对照饮食增加得更快。此外，根茎苷，SGLT1的特异性抑制剂，阻止了HS饮食小鼠空肠葡萄糖转运体表达的增加和肝脏的时相推进。这些结果表明，饮食HS诱导的葡萄糖吸收增加改变了外周分子昼夜节律的食物携带。(C)2010 Elsevier Inc.保留所有权利。
Dietary compounds influence the expression of various genes and play a major role in changing physiological and metabolic states. However, little is known about the role of food ingredients in the regulation of circadian gene expression. Here, we show that feeding mice with a high-salt (HS) diet ad libitum for over 2 weeks advanced the phase of clock gene expression by about 3 h in the liver, kidney, and lung, but did not change circadian feeding, drinking, and locomotor rhythms. Focused DNA microarray analysis showed that the expression phase of many genes related to metabolism in the liver was also advanced. Immediately before phase advancement in peripheral tissues, the mRNA expression of sodium-glucose cotransporter 1 (Sglt1) and glucose transporter 2 (Glut2), that are responsible for glucose absorption, was increased in the jejunum. Furthermore, blood glucose uptake increased more rapidly after consuming the HS diet than the control diet. Moreover, phloridzin, a specific inhibitor of SGLT1, prevented the increased glucose transporter expression in the jejunum and phase advancement in the livers of mice on the HS diet. These results suggest that increased glucose absorption induced by dietary HS alters the food entrainment of peripheral molecular circadian rhythms. (C) 2010 Elsevier Inc. All rights reserved.