Thiamine Assays-Advances, Challenges, and Caveats.
Thiamine Assays-Advances, Challenges, and Caveats.
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DOI：
10.1002/open.201600160
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发表时间：
2017-04
期刊：
影响因子：
2.3
通讯作者：
Kraft CE
中科院分区：
文献类型：
--
作者：
Edwards KA;Tu-Maung N;Cheng K;Wang B;Baeumner AJ;Kraft CE
硫胺素（维生素B1）对所有生物体的健康至关重要，长期以来，缺乏维生素B1与鱼类、鸟类、短吻鳄和家养反刍哺乳动物等动物的疾病有关。硫胺素还涉及几种人类疾病，包括阿尔茨海默氏症、糖尿病、痴呆、抑郁症，最值得注意的是，Wernicke-Korsakoff综合征和脚气病。然而，硫胺素的高灵敏度和特异性检测仍然是一个分析挑战，因为需要分别在环境和人体样品中检测pM至nm水平的硫胺素，需要区分各种磷酸化变体，并且非常需要快速的现场检测。此外，由于包括鱼组织、海水和体液在内的相关样品基质的复杂性，必须进行适当的样品制备。这次审查有两个目标。首先，它提供了一个全面的概述分析技术发表的硫胺素检测在过去的15年。其次，它描述了基于生物识别的分析方法的原理，并可能为快速和高通量的硫胺素分析开辟新的途径。最值得注意的是，周质结合蛋白，核酶和适体是特别感兴趣的，因为它们作为生物亲和识别元件，可以填补一个重要的测定技术空白，由于硫胺素特异性商业抗体的不可用。最后，作者简要评价了主要分析概念的关键成果，并建议创新技术如何帮助开发灵敏和特异的硫胺素分析测试系统。
Thiamine (vitamin B1) is essential to the health of all living organisms and deficiency has long been associated with diseases in animals such as fish, birds, alligators, and domesticated ruminant mammals. Thiamine is also implicated in several human diseases including Alzheimer's, diabetes, dementia, depression and, most notably, Wernicke–Korsakoff syndrome and Beriberi disease. Yet, highly sensitive and specific detection of thiamine remains an analytical challenge, as pM to nm levels of thiamine need to be detected in environmental and human samples, respectively, various phosphorylated variants need to be discriminated, and rapid on‐site detection would be highly desirable. Furthermore, appropriate sample preparation is mandatory, owing to the complexity of the relevant sample matrices including fish tissues, ocean water, and body fluids. This Review has two objectives. First, it provides a thorough overview of analytical techniques published for thiamine detection over the last 15 years. Second, it describes the principles of analytical approaches that are based on biorecognition and may open up new avenues for rapid and high‐throughput thiamine analysis. Most notably, periplasmic binding proteins, ribozymes, and aptamers are of particular interest, as they function as bioaffinity recognition elements that can fill an important assay technology gap, owing to the unavailability of thiamine‐specific commercial antibodies. Finally, the authors provide brief evaluations of key outcomes of the major assay concepts and suggest how innovative techniques could help develop sensitive and specific thiamine analytical test systems.