Diversity and distribution patterns of root-associated fungi on herbaceous plants in alpine meadows of southwestern China
Diversity and distribution patterns of root-associated fungi on herbaceous plants in alpine meadows of southwestern China
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DOI：
10.3852/14-324
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发表时间：
2016-03-01
期刊：
影响因子：
2.8
通讯作者：
Yang, Zhu L.
中科院分区：
文献类型：
--
作者：
Gao, Qian;Yang, Zhu L.
利用根端ITS rDNA序列分析技术，对中国西南高山草甸3个样点的4种外生菌根草本植物（毛叶嵩草Kobresia capillifolia、小苔草Carex parva、大叶蓼Polygonum macrophyllum和委陵菜Potentilla fallens）根际真菌多样性进行了研究。从跨越所有三个位点的四种植物物种中获得了377个分类为154个操作分类单元的真菌序列（ITS之间的序列相似性>= 97%）。对于大多数OTU，在GenBank和/或UNITE中未发现相似的分类群（在GenBank中具有97%的相似性）。真菌操作分类单位（OTUs）中，外生菌根真菌占64%，内生真菌占4%，其余33%为未鉴定的根相关真菌。真菌OTU以57%的担子菌和43%的子囊菌为代表。Inocybe、Tomentella/Thelopora、Sebacina、Hebeloma、Pezizomycotina、Cenococcum geophilum complex、Cortinarius、Lactarius和Helotiales是富含OTU的真菌谱系。在整个网站和主机物种的根相关真菌群落一般表现出较低的主机和网站的特异性，但主机和采样点的偏好。总体而言，我们的研究揭示了值得注意的多样性和特有性的高山植物根相关真菌在这个全球生物多样性热点。
The diversity of root-associated fungi associated with four ectomycorrhizal herbaceous species, Kobresia capillifolia, Carex parva, Polygonum macrophyllum and Potentilla fallens, collected in three sites of alpine meadows in southwestern China, was estimated based on internal transcribed spacer (ITS) rDNA sequence analysis of root tips. Three hundred seventy-seven fungal sequences sorted to 154 operational taxonomical units (sequence similarity of >= 97% across the ITS) were obtained from the four plant species across all three sites Similar taxa (in GenBank with 97% similarity) were not found in GenBank and/or UNITE for most of the OTUs. Ectomycorrhiz a made up 64% of the fungi operational taxonomic units (OTUs), endophytes constituted 4% and the other 33% were unidentified root-associated fungi. Fungal OTUs were represented by 57% basidiomycetes and 43% ascomycetes. Inocybe, Tomentella/Thelophora, Sebacina, Hebeloma, Pezizomycotina, Cenococcum geophilum complex, Cortinarius, Lactarius and Helotiales were OTU-rich fungal lineages. Across the sites and host species the root associated fungal communities generally exhibited low host and site specificity but high host and sampling site preference. Collectively our study revealed noteworthy diversity and endemism of root-associated fungi of alpine plants in this global biodiversity hotspot.