Clonal analysis of meningiomas.
Clonal analysis of meningiomas.
复制标题
脑膜瘤的克隆分析。
DOI：
10.1097/00006123-199606000-00029
复制
发表时间：
1996
期刊：
影响因子：
4.8
通讯作者：
Darras,BT
中科院分区：
文献类型：
--
作者：
Wu,JK;MacGillavry,M;Kessaris,C;Verheul,B;Adelman,LS;Darras,BT
脑膜瘤是由脑膜瘤细胞引起的原发性脑肿瘤。它们通常生长缓慢，并且很容易通过手术与大脑分离。最近的脑膜瘤克隆分析，使用甲基化敏感的限制性片段长度多态性，建议单克隆起源。使用同样的技术，但与高信息量的X染色体探针（M27β），我们发现，17（85%）的20个脑膜瘤分析是信息。在17个信息肿瘤中，8个（47%）是单克隆的，3个（18%）在X染色体上有杂合性丢失，并且出乎意料地，6个（35%）具有多克隆模式。来自一个肿瘤的两个区域的样本分别在X染色体上显示单克隆模式和杂合性丢失。回顾17例肿瘤的组织病理学和放射学特征，并不能区分克隆性肿瘤和多克隆性肿瘤。我们的结论是脑膜瘤是异质性的克隆组成。
MENINGIOMAS ARE PRIMARY brain tumors arising from meningothelial cells. They usually grow slowly and are surgically easy to separate from the brain. A recent clonal analysis of meningiomas, using methylation-sensitive restriction fragment length polymorphisms, suggested a monoclonal origin. Using the same technique but with a highly informative X chromosome probe (M27β), we found that 17 (85%) of the 20 meningiomas analyzed were informative. Of the 17 informative tumors, 8 (47%) were monoclonal, 3 (18%) had loss of heterozygosity on the X chromosome, and, unexpectedly, 6 (35%) had a polyclonal pattern. Samples from two areas of one tumor showed a monoclonal pattern and loss of heterozygosity, respectively, on the X chromosome. A review of the histopathological and radiological features of the 17 informative tumors did not help to distinguish the clonal from the polyclonal tumors. We conclude that meningiomas are heterogeneous in clonal composition.