FINE-STRUCTURE OF CRYPTOCOCCUS-NEOFORMANS GROWN IN-VITRO AS OBSERVED BY FREEZE-ETCHING
FINE-STRUCTURE OF CRYPTOCOCCUS-NEOFORMANS GROWN IN-VITRO AS OBSERVED BY FREEZE-ETCHING
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DOI：
10.1128/jb.113.3.1442-1448.1973
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发表时间：
1973-01-01
影响因子：
3.2
通讯作者：
NISHIURA, M
中科院分区：
文献类型：
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作者：
TAKEO, K;UESAKA, I;NISHIURA, M
通过冷冻蚀刻技术观察在培养基上生长的新型隐球菌。在胶囊中，冷冻蚀刻时可以看到短原纤维。该生物体的细胞壁外观独特，呈现出两层。外层颗粒密集，直径约20 nm，而内层颗粒稀疏。该生物体的细胞膜外观根据培养基的不同而明显不同。当在甘油培养基上生长时，与其他酵母一样，细胞膜具有透明但稍长且弯曲的内陷。然而，在非甘油培养基上生长的细胞膜仅表现出一些不规则形状的内陷。相反，该生物体的特征是，细胞膜显示出直径为 40 至 200 nm 的圆形凹陷，这是旁壁体的表面视图。在交叉断裂的细胞中，发现了两种类型的旁体。其中一些含有直径约 50 nm 的单个囊泡。这些似乎在分泌细胞质囊泡中发挥作用。还获得了表明细胞质中存在多泡体和多泡胞质体的数据。一些袋状旁壁体显示出多层膜。这些被认为是质膜体。该生物体在其他方面与报道的其他酵母相似。
Cryptococcus neoformansgrown on culture media was observed by the freeze-etching technique. In the capsule, short fibrils were seen when freezeetched. This organism was unique in the appearance of the cell wall, which showed two strata. The outer one was dense with particles of about 20 nm in diameter, whereas the inner one was sparse in particles. The appearance of the cell membrane of this organism differed distinctly depending on the culture media. When grown on glycerol medium, the cell membrane possessed, as do other yeasts, clear but somewhat longer and curved invaginations. The membrane of cells grown on nonglycerol medium exhibited, however, only a few invaginations of irregular shape. Instead, characteristically of this organism, the cell membrane showed round depressions of 40 to 200 nm in diameter which were the surface view of the paramural bodies. In cross-fractured cells, both types of paramural bodies were found. Some of them contained a single vesicle of about 50 nm in diameter. These seem to play a role in secreting the cytoplasmic vesicles. Data suggesting the existence of multivesicular bodies in the cytoplasm and multivesicular lomasomes were also obtained. Some of the baglike paramural bodies showed multilayered membrane. These are thought to be plasmalemmasomes. This organism was similar to other yeasts reported in other respects.