Age-related changes in the human retina
Age-related changes in the human retina
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DOI：
10.1016/s0008-4182(04)80054-1
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发表时间：
2004-02-01
影响因子：
4.2
通讯作者：
Feher, J
中科院分区：
文献类型：
--
作者：
Cavallotti, C;Artico, M;Feher, J
背景：在先前的研究中，扫描电子显微镜（SEM）显示大鼠视网膜的年龄相关性变化。我们进行了一项研究，以评估与年龄相关的变化，在人类retina.Methods：新鲜视网膜组织的样本从年轻（年龄22岁或以下）和老年人（年龄66岁或以上）捐助者进行了研究，通过传统的组织学方法和扫描电镜。取4例角膜移植术后供体的8个视网膜和4例外伤性眼球摘除术后供体的4个视网膜，进行图像定量分析。视网膜组织匀浆中的细胞质（游离）和结构（组织相关）蛋白质的浓度测定通过生化方法。结果：有一个下降，在所有功能的研究与结构蛋白质的浓度除外。年轻受试者的平均视网膜厚度（和平均值的标准误差）为426（34.2）mum，老年受试者为261（18.9）mum。神经节细胞平均数（和平均值的标准误差）为413.5/mm（2）（32.3/mm（2））和256.2/mm（2））（26.8/mm（2）），毛细管3.6/mm（2）（1.4/mm（2））和1.8/mm（2）（1.2/mm（2）），突触体122.4（4.9）常规单位/观测面积和38.5（1.6）分别观察到的细胞突起的CU/面积82.3细胞间连接分别为36.4（2.5）CU/面积和14.3（1.4）CU/面积。每毫克新鲜组织中总蛋白的平均浓度（和平均值的标准误差）为92.1青年组为1.8，（1.3）年龄较大的受试者出现mug;细胞质蛋白的相应值分别为27.6（1.3）μ g和11.8（0.8）μ g，结构蛋白的相应值分别为64.4（1.6）μ g和86.9（1.4）μ g。年轻人和老年人受试者之间的所有差异都是显着的（p < 0.001），除了细胞质和结构蛋白的平均浓度。扫描电镜提供了感光细胞、双极细胞和神经节细胞年龄相关变化的新的形态计量学信息，增加了我们对这一主题的理解。我们的研究结果可以作为一个模型或正常值时，研究可能发生在人类视网膜的病理条件下的其他变化。
Background: In a previous study, scanning electron microscopy (SEM) showed agerelated changes in the rat retina. We carried out a study to evaluate age-related changes in the human retina.Methods: Samples of fresh retinal tissue obtained from younger (age 22 years or less) and older (age 66 years or more) donors were studied by means of traditional histologic methods and by SEM. Eight retinas were obtained from four donors whose corneas had been used for transplantation, and four retinas were obtained from four subjects whose eyes had been enucleated owing to injury.All morphologic results were subjected to quantitative analysis of images. The concentration of cytoplasmic (free) and structural (tissue-associated) protein in retinal tissue homogenates was determined by means of biochemical methods.Results: There was a decrease in all features studied with the exception of structural protein concentration. The mean retinal thickness (and standard error of the mean) was 426 (34.2) mum in the younger subjects and 261 (18.9) mum in the older subjects. The mean numbers of ganglion cells (and standard error of the mean) were 413.5/mm(2) (32.3/mm(2)) and 256.2/mm(2) (26.8/mm(2)) respectively, of capillaries 3.6/mm(2) (1.4/mm(2)) and 1.8/mm(2) (1.2/mm(2)) respectively, of synaptic bodies 122.4 (4.9) conventional units (CU)/area observed and 38.5 (1.6) CU/area observed respectively, of cellular processes 82.3 (3.1) CU/area observed and 13.1 (1.5) CU/ area observed respectively, and of intercellular connections 36.4 (2.5) CU/area observed and 14.3 (1.4) CU/area observed respectively. The mean concentration of total protein per milligram of fresh tissue (and standard error of the mean) was 92.1 (1.8) mug in the younger subjects and 78.7 (1.3) mug in the older subjects; the corresponding values for cytoplasmic protein were 27.6 (1.3) mug and 11.8 (0.8) mug, and for structural protein, 64.4 (1.6) mug and 86.9 (1.4) mug. All differences between the younger and older subjects were significant (p < 0.001) with the exception of mean concentration of cytoplasmic and of structural protein.Interpretation: The human retina undergoes specific changes with aging. SEM provides new morphometric information regarding age-related changes in photoreceptor cells, bipolar cells and ganglion cells that increases our understanding of this topic. Our results may be adopted as a model or as normal values when studying other changes that may occur in the human retina in pathological conditions.