An immunohistochemical study of androgen, oestrogen and progesterone receptors in the vulva and vagina
An immunohistochemical study of androgen, oestrogen and progesterone receptors in the vulva and vagina
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DOI：
10.1111/j.1471-0528.1998.tb10056.x
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发表时间：
1998-02-01
期刊：
影响因子：
--
通讯作者：
MacLean, AB
中科院分区：
文献类型：
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作者：
Hodgins, MB;Spike, RC;MacLean, AB
目的了解性类固醇在人体外阴的作用部位。方法采用雄激素、雌激素和孕激素受体单克隆抗体对外阴皮肤、阴道和耻骨上皮肤冷冻切片进行染色。设计了一个评分系统来比较皮肤表皮和真皮与阴道上皮和间质中的受体分布。结果在皮肤表皮角质形成细胞、皮脂腺、汗腺、毛囊、真皮成纤维细胞、阴道上皮细胞和间质成纤维细胞中可见雄激素受体。大阴唇和小阴唇表皮的雄激素受体评分明显高于阴道上皮。雌激素受体存在于阴道上皮和小阴唇表皮的基底细胞和基底上细胞中，而在真皮肤中仅存在于基底角质形成细胞中。在间质成纤维细胞、阴道平滑肌和皮肤真皮成纤维细胞中可见。雌激素受体在阴道上皮和间质中最高，在耻骨上皮肤中最低。阴道上皮、成纤维细胞和平滑肌中可见黄体酮受体，但外阴中未见。绝经前或绝经后妇女的外阴雄激素或雌激素受体染色没有明显差异。结论从阴道到外阴的过渡表现为雄激素升高，雌激素和孕激素受体降低。这种受体的分布表明雌激素乳膏在外阴上的作用有限。
Objective To map potential sites of sex steroid action In the human vulva.Methods Monoclonal antibodies to androgen, oestrogen and progesterone receptors were used to stain frozen sections of vulval skin, vagina and suprapubic skin. A scoring system was devised to compare receptor distribution in the epidermis and dermis of skin with vaginal epithelium and stroma.Results Androgen receptors were seen in epidermal keratinocytes, sebaceous glands, sweat glands, hair follicles and dermal fibroblasts of skint and epithelial cells and stromal fibroblasts of the vagina. Androgen receptor scores were significantly higher in the epidermis of labia majora and minora than in vaginal epithelium. Oestrogen receptors were seen in basal and suprabasal cells of vaginal epithelium and epidermis of labia minora but were restricted to basal keratinocytes in true skin. They were seen in stromal fibroblasts and vaginal smooth muscle, and dermal fibroblasts of the skin. Oestrogen receptors were highest in vaginal epithelium and stroma, and lowest in suprapubic skin. Progesterone receptors were seen in vaginal epithelium, fibroblasts and smooth muscle but not in the vulva. There was no evidence of significant differences in androgen or oestrogen receptor staining in the vulva of pre- or postmenopausal women.Conclusion The transition from vagina to vulva is marked by an increase in androgen and a decrease in oestrogen and progesterone receptors. This distribution of receptors would indicate a limited role for oestrogen creams on the vulva.