CORNEA-SPECIFIC EXPRESSION OF K12-KERATIN DURING MOUSE DEVELOPMENT
CORNEA-SPECIFIC EXPRESSION OF K12-KERATIN DURING MOUSE DEVELOPMENT
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DOI：
10.3109/02713689309029222
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发表时间：
1993-11-01
影响因子：
2
通讯作者：
KAO, WWY
中科院分区：
文献类型：
--
作者：
LIU, CY;ZHU, G;KAO, WWY
小鼠K12角蛋白的全长cDNA的特征在于通过测序从小鼠角膜cDNA文库中分离的重叠cDNA克隆。使用北方印迹杂交，放射性标记的cDNA杂交到一个1.9 kb的mRNA从成人角膜，但不从其他小鼠组织，包括口鼻部，食道，舌头，和皮肤。在小鼠发育过程中，角膜不表达K12 mRNA，直到出生后4天，当上皮开始分层判断北方印迹和原位杂交。原位杂交与H-3标记的cDNA探针和免疫组化研究与抗体的合成寡肽推导出兔K12的cDNA证明，这种小鼠K12角蛋白表达在所有细胞层的成人角膜上皮，和基底层，但不是基底层的角膜缘上皮。表皮生长因子（EGF）已被证明可以促进体外培养的鸡和人角膜上皮分层。为了研究EGF是否可以促进K12的表达，将EGF给予新生小鼠。结果表明，EGF延缓角膜上皮细胞K12的表达，即使它促进角膜上皮分层在小鼠发育过程中。两者合计，我们的研究结果表明，K12角蛋白的表达是角膜特异性的，分化依赖性的，发育调节。
The full-length cDNA of mouse K12 keratin was characterized by sequencing overlapping cDNA clones isolated from a mouse cornea cDNA library. Using Northern blot hybridization, the radio-labeled cDNA hybridized to a 1.9 kb mRNA from adult cornea, but not from other mouse tissues including snout, esophagus, tongue, and skin. During mouse development, corneas do not express K12 mRNA until 4 days postnatal when the epithelium begins to stratify as judged by Northern blot and in situ hybridization. In situ hybridization with H-3-labeled cDNA probe and immunohistochemical studies with antibodies against a synthetic oligo-peptide deduced from rabbit K12 cDNA demonstrate that this mouse K12 keratin is expressed in all cell layers of adult corneal epithelium, and the suprabasal layers, but not the basal layer of the limbal epithelium. Epidermal growth factor (EGF) has been shown to promote epithelium stratification of cultured chicken and human corneas in vitro. To examine whether EGF can promote K12 expression, EGF was administered to neonatal mice. The results indicate that EGF retards K12 expression by corneal epithelial cells, even though it promotes corneal epithelial stratification during mouse development. Taken together, our results demonstrate that the expression of K12 keratin is cornea-specific, differentiation-dependent, and developmentally regulated.