Fibroblasts from women with pelvic organ prolapse show differential mechanoresponses depending on surface substrates
Fibroblasts from women with pelvic organ prolapse show differential mechanoresponses depending on surface substrates
复制标题
DOI：
10.1007/s00192-013-2069-z
复制
发表时间：
2013-09-01
影响因子：
1.8
通讯作者：
Helder, Marco N.
中科院分区：
文献类型：
--
作者：
Ruiz-Zapata, Alejandra M.;Kerkhof, Manon H.;Helder, Marco N.
在盆腔器官脱垂（POP）的病理生理学和治疗背景下，对动态细胞-基质相互作用知之甚少。本研究旨在确定不同程度脱垂妇女的成纤维细胞对机械刺激和基质的反应差异，将来自POP定量（POP-Q）系统0、II和IV期三名患者阴道壁的成纤维细胞在涂有或未涂有胶原I的人工聚合物基质上拉伸。在蛋白质和基因表达水平上评价了形态学和影响基质重塑的合成代谢/分解代谢化合物的变化。采用单因素方差分析（ANOVA）进行统计学分析，然后进行Tukey-Kramer事后检验。与健康成纤维细胞相比，POP成纤维细胞在酶和基因表达水平上显示出延迟的细胞排列和对细胞外基质重塑因子的较低反应。与健康细胞相比，POP成纤维细胞在两种人工聚合物基质上显示出差异的机械反应。在设计或改进用于治疗POP的植入物时应考虑到这一点。
Little is known about dynamic cell-matrix interactions in the context of pathophysiology and treatments for pelvic organ prolapse (POP). This study sought to identify differences between fibroblasts from women with varying degrees of prolapse in reaction to mechanical stimuli and matrix substrates in vitro.Fibroblasts from the vaginal wall of three patients with POP Quantification (POP-Q) system stages 0, II, and IV were stretched on artificial polymer substrates either coated or not coated with collagen I. Changes in morphology and anabolic/catabolic compounds that affect matrix remodelling were evaluated at protein- and gene-expression levels. Statistical analysis was performed using one-way analysis of variance (ANOVA), followed by Tukey-Kramer's post hoc test.POP fibroblasts show delayed cell alignment and lower responses to extracellular matrix remodelling factors at both enzymatic- and gene-expression levels compared with healthy fibroblasts.POP fibroblasts, when compared with healthy cells, show differential mechanoresponses on two artificial polymer substrates. This should be taken into account when designing or improving implants for treating POP.