The Mouse Forced Swim Test
The Mouse Forced Swim Test
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DOI：
10.3791/3638
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发表时间：
2012-01-01
影响因子：
1.2
通讯作者：
Gould, Todd D.
中科院分区：
文献类型：
--
作者：
Can, Adem;Dao, David T.;Gould, Todd D.
强迫游泳试验是一种啮齿动物行为试验，用于评价抗抑郁药物、新化合物的抗抑郁功效以及旨在呈现或预防抑郁样状态的实验操作。将小鼠置于充满水的不可逃脱的透明罐中，并测量它们的逃脱相关的移动行为。强迫游泳测试是简单可靠的，它需要最少的专业设备。强迫游泳测试的成功实施需要遵守某些程序细节并最大限度地减少对小鼠的不必要的压力。在方案描述和随附的视频中，我们解释了如何进行该测试的小鼠版本，重点是可能不利于结果解释的潜在陷阱以及如何避免它们。此外，我们解释了如何在测试中表现出的行为进行评估。
The forced swim test is a rodent behavioral test used for evaluation of antidepressant drugs, antidepressant efficacy of new compounds, and experimental manipulations that are aimed at rendering or preventing depressive-like states. Mice are placed in an inescapable transparent tank that is filled with water and their escape related mobility behavior is measured. The forced swim test is straightforward to conduct reliably and it requires minimal specialized equipment. Successful implementation of the forced swim test requires adherence to certain procedural details and minimization of unwarranted stress to the mice. In the protocol description and the accompanying video, we explain how to conduct the mouse version of this test with emphasis on potential pitfalls that may be detrimental to interpretation of results and how to avoid them. Additionally, we explain how the behaviors manifested in the test are assessed.