EFFECTS OF EXTRACELLULAR IONIZED CALCIUM, DILTIAZEM AND CAMP ON MOTILITY OF HUMAN-SPERMATOZOA
EFFECTS OF EXTRACELLULAR IONIZED CALCIUM, DILTIAZEM AND CAMP ON MOTILITY OF HUMAN-SPERMATOZOA
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DOI：
10.1016/s0022-5347(17)41225-0
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发表时间：
1989-05-01
影响因子：
6.6
通讯作者：
RAJFER, J
中科院分区：
文献类型：
--
作者：
AABERG, RA;SAUER, MV;RAJFER, J
为了阐明钙离子和钙通道阻滞剂对人精子运动的影响，进行了体外研究，观察了外源性钙、乙二胺四乙酸（EDTA）、地尔硫卓和二丁酰环磷酸腺苷（db-cAMP）对人精子运动百分率和平均运动速度的影响。将来自21个可育供体的洗涤精子与离子钙（0、5、10和50 mM）、EDTA（1和10 mM）、地尔硫卓（1、10、100 μ M和1 mM）和cAMP（5 mM）一起孵育。在10分钟和120分钟时，通过计算机分析视频显微照片确定运动百分比和平均速度。在宽范围的细胞外离子钙浓度（50 μ M至50 mM）下，人精子的百分比运动性和平均速度不受刺激或抑制。在离子钙浓度
In order to elucidate the effects of ionized calcium and calcium channel blockers on human sperm motility, an in vitro study was conducted looking at the effects of exogenous calcium, ethylene diamine tetraacetic acid (EDTA), diltiazem and dibutyrl cyclic adenosine monophosphate (db-cAMP) on per cent motility and mean velocity of human sperm. Washed spermatozoa from 21 fertile donors were incubated with ionized calcium (zero, five, 10 and 50 mM), EDTA (one and 10 mM), diltiazem (one, 10, 100 .mu.M and one mM) and cAMP (five mM). Per cent motility and mean velocity determinations were made at 10 and 120 min. by computerized analysis of videomicrographs. Over a wide range of extracellular ionized calcium concentrations (50 .mu.M to 50 mM), per cent motility and mean velocity of human sperm were not stimulated or inhibited. At ionized calcium concentration