A concise guide to cDNA microarray analysis
A concise guide to cDNA microarray analysis
复制标题
DOI:
10.2144/00293bi01
复制
发表时间:
2000-09-01
期刊:
影响因子:
2.7
通讯作者:
Quackenbush, J
中科院分区:
文献类型:
--
作者:
Hegde, P;Qi, R;Quackenbush, J
Microarray expression analysis has become one of the most widely used functional genomics tools. Efficient application of this technique requires the development of robust and reproducible protocols. We have optimized all aspects of the process, including PCR amplification of target cDNA clones, microarray printing, probe labeling and hybridization, and have developed strategies for data normalization and analysis.