Spectroscopic studies on the binding interaction of phenothiazinium dyes, azure A and azure B to double stranded RNA polynucleotides
Spectroscopic studies on the binding interaction of phenothiazinium dyes, azure A and azure B to double stranded RNA polynucleotides
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DOI：
10.1016/j.saa.2015.07.091
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发表时间：
2016-01-05
影响因子：
4.4
通讯作者：
Kumar, Gopinatha Suresh
中科院分区：
文献类型：
--
作者：
Khan, Asma Yasmeen;Kumar, Gopinatha Suresh
本文报道了吩噻嗪染料天青A和天青B与双链核糖核酸poly（A）、poly（U）、poly（C）、poly（G）和poly（I）、poly（C）相互作用的光谱特性。吸光度和荧光研究表明，这些染料与RNA的结合亲和力分别为10（6）M-1与poly（A）.poly（U），10（5）M-1与poly（C）.poly（G）和poly（I）.poly（C）。荧光猝灭和粘度数据提供了确凿的证据，这些RNA双链体的染料嵌入。圆二色性结果表明，RNA的构象受到扰动的相互作用和染料获得强烈的诱导光学活性的结合。天青B与三种RNA的结合能力均强于天青A，其结合能力为poly（A）、poly（U）> poly（C）、poly（G）> poly（I）、poly（C）。(C)2015 Elsevier B. V.版权所有。
This manuscript presents spectroscopic characterization of the interaction of two phenothiazinium dyes, azure A and azure B with double stranded (ds) ribonucleic acids, poly(A).poly(U), poly(C).poly(G) and poly(I).poly(C). Absorbance and fluorescence studies revealed that these dyes bind to the RNAs with binding affinities of the order 10(6) M-1 to poly(A).poly(U), and 10(5) M-1 to poly(C).poly(G) and poly(I).poly(C), respectively. Fluorescence quenching and viscosity data gave conclusive evidence for the intercalation of the dyes to these RNA duplexes. Circular dichroism results suggested that the conformation of the RNAs was perturbed on interaction and the dyes acquired strong induced optical activity on binding. Azure B bound to all the three RNAs stronger than azure A and the binding affinity varied as poly(A).poly(U) > poly(C).poly(G) > poly(I).poly(C) for both dyes. (C) 2015 Elsevier B.V. All rights reserved.