A unique neurogenomic state emerges after aggressive confrontations in males of the fish Betta splendens
A unique neurogenomic state emerges after aggressive confrontations in males of the fish Betta splendens
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雄性斗鱼在发生攻击性对抗后会出现一种独特的神经基因组状态
DOI：
10.1016/j.gene.2021.145601
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发表时间：
2021
期刊：
影响因子：
3.5
通讯作者：
Okada Norihiro
中科院分区：
文献类型：
--
作者：
Vu Trieu-Duc;Iwasaki Yuki;Oshima Kenshiro;Chiu Ming-Tzu;Nikaido Masato;Okada Norihiro
领土防御涉及与竞争对手频繁的侵略性对抗，但对于竞争领土建立的个体之间脑转录组谱的变化知之甚少。我们之前的研究表明，当两条斗鱼相互作用时，它们大脑中的转录组同步，这反映了它们在基因表达水平上的相互评估过程。在这里，我们的目标是评估对手的大脑转录组特征在改变他们的社会地位后（即，胜利者/失败者已经出现）是如何立即改变的，以及在这种改变后30分钟。我们发现某些基因的表达变化在不同的战斗阶段是独特的，某些基因的表达模式在所有战斗阶段都是短暂或持续改变的。这些大脑转录组反应与战斗中的行为变化是一致的。引人注目的是，在战斗结束后，一对在战斗中大脑转录组同步的特异性逐渐丧失，导致基础神经基因组状态的出现，在这种状态下，基因表达的变化减少到最小，并在所有个体中保持一致。这种状态与冬眠状态有共同的特点，动物采用冬眠状态来降低代谢率以节省能量。有趣的是，与新陈代谢、自闭症谱系障碍和长期记忆相关的基因的表达变化仍然将输家和赢家区分开来。在一起，战斗系统使用maleB。splendenss为研究脊椎动物攻击的神经基因组状态提供了一个很有前途的平台。
Territorial defense involves frequent aggressive confrontations with competitors, but little is known about how brain-transcriptomic profiles change between individuals competing for territory establishment. Our previous study elucidated that when two fishBetta splendensmales interact, transcriptomes across their brains synchronize in a way that reflects a mutual assessment process between them at the gene expression level. Here we aim to evaluate how the brain-transcriptomic profiles of opponents change immediately after shifting their social status (i.e., the winner/loser has emerged) and 30 min after this shift. We showed that changes in the expression of certain genes are unique to different fighting stages and the expression patterns of certain genes are transiently or persistently changed across all fighting stages. These brain transcriptomic responses are in accordance with behavioral changes across the fight. Strikingly, the specificity of the brain-transcriptomic synchronization of a pair during fighting was gradually lost after fighting ceased, leading to the emergence of a basal neurogenomic state in which the changes in gene expression were reduced to minimum and consistent across all individuals. This state shares common characteristics with the hibernation state that animals adopt to minimize their metabolic rates to save energy. Interestingly, expression changes for genes related to metabolism, autism spectrum disorder, and long-term memory still differentiated losers from winners. Together, the fighting system using maleB. splendensprovides a promising platform for investigating neurogenomic states of aggression in vertebrates.