Type 2 heat-labile enterotoxin (LT-II)-producing Escherichia coli isolated from ostriches with diarrhea
Type 2 heat-labile enterotoxin (LT-II)-producing Escherichia coli isolated from ostriches with diarrhea
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DOI：
10.1016/j.vetmic.2004.11.005
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发表时间：
2005-02-25
影响因子：
3.3
通讯作者：
Yano, T
中科院分区：
文献类型：
--
作者：
Nardi, ARM;Salvadori, MR;Yano, T
从巴西腹泻鸵鸟中分离的大肠杆菌培养上清液可引起Vero细胞延长、中国仓鼠卵巢（CHO）细胞圆缩和鸵鸟胚胎成纤维细胞（OEF）细胞质空泡形成，但对鸡胚胎成纤维细胞（CEF）无细胞毒性。这些作用不能被抗霍乱毒素血清所中和。聚合酶链反应实验表明，鸵鸟大肠杆菌含有eltII-A基因编码，但不含1型热不稳定肠毒素（eltA）、热不稳定肠毒素（estA、estB）、细胞毒素（stx-I、stx-II）或细胞毒性坏死因子（cnf2、cnf2）编码基因。所有分离株均为O15:H8血清型。LT-II在鸵鸟腹泻中的肠致病性相关性仍未确定。(C) 2004 Elsevier B.V.版权所有
The culture supernatant of Escherichia coli, isolated from ostriches with diarrhea in Brazil, caused elongation in Vero cell, rounding in Chinese hamster ovary (CHO) cells and a cytoplasmic vacuolation in ostrich embryo fibroblasts (OEF), but it was not cytotoxic for chicken embryo fibroblasts (CEF). These effects were not neutralized by antiserum to cholera toxin. Polymerase chain reaction assays showed that the ostrich E.coli contained the gene encoding (eltII-A), but not those for type 1 heat-labile enterotoxin (eltA), heat-stable enterotoxins (estA, estB), verocytotoxins (stx-I, stx-II), or cytotoxic necrotizing factors (cnf 1, cnf 2). All isolates belonged to serotype O15:H8. The enteropathogenic relevance of LT-II in ostrich diarrhea remains undetermined. (C) 2004 Elsevier B.V. All rights reserved.