Measurement of MicroRNA
Measurement of MicroRNA
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DOI：
10.1177/1099800411430380
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发表时间：
2013-04
影响因子：
2.5
通讯作者：
E. Flowers;E. Froelicher;B. Aouizerat
中科院分区：
文献类型：
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作者：
E. Flowers;E. Froelicher;B. Aouizerat
微小RNA（miRs）是多肽信使RNA（mRNA）表达的表观遗传调节因子。因此，miR代表了一种有趣的机制，通过该机制，假设基因-环境相互作用发生在基因表达的表观遗传控制水平上。除了来自体外研究的有希望的发现表明miR具有作为治疗剂在改变病理生理条件的过程中起作用的潜力之外，最近的人类研究揭示了响应于行为干预的miR表达模式的变化。作者概述了miR是如何从其他遗传物质中保存和分离的，并描述了在研究环境中测量miR的常用方法，包括北方印迹、聚合酶链反应和微阵列。作者还介绍了分析高通量miR表达的生物信息学方法和用于创建miR-mRNA结合预测模型的技术，以描述受特定miR影响的可能生理途径。
MicroRNAs (miRs) are epigenetic regulators of messenger RNAs' (mRNA) expression of polypeptides. As such, miRs represent an intriguing mechanism by which gene–environment interactions are hypothesized to occur on the level of epigenetic control over gene expression. In addition to promising findings from in vitro studies indicating that miRs have the potential to function as therapeutic agents in modifying the course of pathophysiologic conditions, recent human studies revealed changes in miR expression patterns in response to behavioral interventions. The authors provide an overview of how miRs are preserved and isolated from other genetic material and describe commonly used methods for measuring miR in the research setting, including Northern blot, polymerase chain reaction, and microarray. The authors also introduce bioinformatic approaches to analysis of high-throughput miR expression and techniques used to create predictive models of miR-mRNA binding to describe possible physiologic pathways affected by specific miRs.