Acute rejection of cardiac allografts by noncytolytic CD4(+) T cell populations.
Acute rejection of cardiac allografts by noncytolytic CD4(+) T cell populations.
复制标题
非溶细胞性 CD4(+) T 细胞群对心脏同种异体移植物的急性排斥。
DOI：
10.1097/00007890-199607270-00026
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发表时间：
1996
期刊：
影响因子：
6.2
通讯作者：
Orosz,CG
中科院分区：
文献类型：
--
作者：
VanBuskirk,AM;Wakely,ME;Orosz,CG
通过将纯化的Balb/c CD 4 + T细胞与经辐照的C57 B1/6（B6）脾细胞加抗IL-4 mAb一起培养来体外产生细胞溶解性T细胞。通过用经辐照的B6脾细胞加重组鼠IL-4培养纯化的Balb/c CD 4 + T细胞，类似地产生匹配的非细胞毒性T细胞。后者的T细胞显示细胞溶解活性，即使在凝集素介导的裂解试验，但产生的特征性细胞因子接触后，与特定的同种异体抗原。将溶细胞T细胞群输注到携带B6心脏同种异体移植物的Balb/c SCID小鼠中，在5至10天内导致急性同种异体移植物排斥反应。将非细胞溶解性T细胞群输注到携带B6心脏同种异体移植物的Balb/c SCID小鼠中也会在7至10天内导致急性同种异体移植物排斥反应。胶原酶消化后从排斥的同种异体移植物中回收的浸润细胞的有限稀释分析（LDA）表明，在整个排斥过程中，CD 4 + T细胞在体内保留了其溶细胞或非溶细胞的功能表型。这些数据表明，分离的CD 4 + T细胞群可以促进快速的急性心脏同种异体移植物排斥反应，并且细胞溶解活性不是这种急性排斥反应所必需的。
Cytolytic T cells were generated in vitro by culturing purified Balb/c CD4+ T cells with irradiated C57B1/6 (B6) splenocytes plus anti-IL-4 mAb. Matched, noncytotoxic T cells were similarly generated by culturing purified Balb/c CD4+ T cells with irradiated B6 splenocytes plus recombinant murine IL-4. The latter T cells displayed to cytolytic activity, even in lectin-mediated lysis assays, but produced characteristic cytokines upon contact with specific alloantigens. Transfusion of cytolytic T cell populations into Balb/c SCID mice bearing B6 cardiac allografts resulted in acute allograft rejection within 5 to 10 days. Transfusion of noncytolytic T cell populations into Balb/c SCID mice bearing B6 cardiac allografts also resulted in acute allograft rejection within 7 to 10 days. Limiting dilution analysis (LDA) of infiltrating cells recovered from rejected allografts after collagenase digestion demonstrated that the CD4+ T cells retained their cytolytic or noncytolytic functional phenotypes in vivo throughout the rejection process. These data demonstrate that isolated CD4+ T cell populations can promote rapid acute cardiac allograft rejection, and that cytolytic activity is not necessary for this acute rejection response.