EXPRESSION OF THE HELIX-LOOP-HELIX GENE ID3 DURING MURINE EMBRYONIC-DEVELOPMENT
EXPRESSION OF THE HELIX-LOOP-HELIX GENE ID3 DURING MURINE EMBRYONIC-DEVELOPMENT
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DOI：
10.1002/aja.1002030205
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发表时间：
1995-06-01
影响因子：
2.5
通讯作者：
WEITH, A
中科院分区：
文献类型：
--
作者：
ELLMEIER, W;WEITH, A
显性-负向螺旋-环-螺旋(DnHLH)转录调控因子家族由四个已知的哺乳动物基因组成：ID1、ID2、ID3(ID3以前被命名为HLH462或HEIR1或HLH1R21)和ID4。我们对ID3基因在小鼠胚胎中的表达进行了详细的研究，以深入了解该基因可能的发育控制功能，并将其与其他哺乳动物dnHLH基因的表达进行比较。通过对妊娠E9.5-17.5的小鼠胚胎切片和新生儿头部进行RNA原位杂交，我们普遍在早期检测到高丰度的转录本，并在大多数位置逐渐下降到E15.5。在胚胎中，ID3在脑、脊髓、嗅觉系统、颧弓、肢体、硬化症、心内膜垫、肠外膜、肺、视网膜、肾集合系统和牙周组织中均有表达。虽然在大多数组织中，mRNA的丰度在后期有所下降，但在牙齿和肾脏中仍保持较高水平。这种表达模式表明，ID3在未分化的组织和处于分化过程中的器官中都有功能。当与其他dnHLH基因的表达进行比较时，很明显，ID3的模式与ID1的模式主要一致。这可能反映了基因功能的部分冗余。此外，先前的实验表明，原癌基因N-myc和ID3存在互斥表达。本研究的结果证实了这些数据。(C)1995年Wiley-Liss公司
The family of dominant-negative helix-loop-helix (dnHLH) transcriptional modulators consists of four mammalian genes known to date: Id1, Id2, Id3 (Id3 was formerly designated HLH462 or HEIR1 or HLH1R21), and Id4. We have performed a detailed study of Id3 expression in mouse embryos in order to gain insight into the possible developmental control functions of this gene and to compare its expression to that of other mammalian dnHLH genes. Employing RNA in situ hybridization on sectioned mouse embryos of gestational stages E9.5-17.5 and neonatal head, we generally detected a high abundance of transcripts at early stages that gradually declined at most sites toward E15.5. Expression of Id3 in embryos was detected in brain, spinal cord, olfactory system, branchial arches, limbs, sclerotome, endocardiac cushions, the outer lining of the gut, lung, retina, the collecting system of the kidney, and in tooth anlagen. Although the abundance of mRNA decreased toward later stages in most tissues, it remained high in teeth and kidney. This expression pattern suggests that Id3 functions both in undifferentiated tissues and in organs which are in the process of differentiation. When compared to the expression of other dnHLH genes, it becomes obvious that the pattern of Id3 mainly coincides with that of Id1. This may reflect a partial redundancy in gene function. Furthermore, previous experiments suggested a mutually exclusive expression of the proto-oncogene N-myc an Id3. The results of the present study confirm these data. (C) 1995 Wiley-Liss, Inc.