Role of Anopheles quadrimaculatus and Coquillettidia perturbans (Diptera: Culicidae) in the transmission cycle of Cache Valley Virus (Bunyaviridae: Bunyavirus) in the Midwest, USA
Role of Anopheles quadrimaculatus and Coquillettidia perturbans (Diptera: Culicidae) in the transmission cycle of Cache Valley Virus (Bunyaviridae: Bunyavirus) in the Midwest, USA
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DOI：
10.1093/jmedent/35.5.660
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发表时间：
1998-09-01
影响因子：
2.1
通讯作者：
Grimstad, PR
中科院分区：
文献类型：
--
作者：
Blackmore, CGM;Blackmore, MS;Grimstad, PR
测试了中西部群体的扰动Coquillettidia（步行者）和四斑按蚊（Say）传播Cache Valley病毒（CV）的能力，CV是一种公认的人类和动物病原体。对现场收集的蚊子喂食含有5.2-6.2 log（10）pfu/ml CV的人工血粉。在28 ℃下培养9-23 d后，75-93%的血饲Cq. perturbans传播感染，6-62%的人将病毒传播给乳鼠。然而，当感染较低的病毒滴度（3.3 log（10）pfu/ml）时，只有10-36%的蚊子传播感染，0-10%的蚊子将病毒传播给乳鼠。在Cq中观察到类似的感染率（21%）。以病毒血症（3.2 log（10）pfu/ml）仓鼠喂养的perturbans。一个.在培养16-23天后，两种剂量均感染了四斑海蜇（81-100%），传播率范围为13-67%。实验室菌株An的传播率。在孵育7-14天后，四斑小蠊萨凡纳的范围为20 - 33%。数据显示，虽然。quadramaculatus比Cq更容易受到CV感染。但令人不安的是，这两种蚊子都可能参与了该病毒在中西部的传播周期。
Midwestern populations of Coquillettidia perturbans (Walker) and Anopheles quadrimaculatus (Say) were tested for their ability to transmit Cache Valley virus (CV), a recognized human and animal pathogen. Field-collected mosquitoes were fed artificial blood meals containing 5.2-6.2 log(10) pfu/ml of CV. After 9-23 d at 28 degrees C, 75-93% of blood-fed Cq. perturbans had disseminated infections and 6-62% transmitted the virus to suckling mice. However, when infected with a lower virus titer (3.3 log(10) pfu/ml), only 10-36% of the mosquitoes had disseminated infections and 0-10% transmitted the virus to suckling mice. A similar infection rate (21%) was observed in Cq. perturbans fed on viremic (3.2 log(10) pfu/ml) hamsters. An. quadrimaculatus were infected (81-100%) by both doses used, with transmission rates ranging from 13-67% after 16-23 d of incubation. Transmission rates for the laboratory strain An. quadrimaculatus SAVANNAH ranged from 20 to 33% after 7-14 d of incubation. Our data show that although An. quadrimaculatus is more susceptible to CV infections than Cq. perturbans, both mosquito species could be involved in the midwestern transmission cycle of the virus.