Highly Enhanced Affinity of Multidentate versus Bidentate Zwitterionic Ligands for Long-Term Quantum Dot Bioimaging
Highly Enhanced Affinity of Multidentate versus Bidentate Zwitterionic Ligands for Long-Term Quantum Dot Bioimaging
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DOI：
10.1021/la302896x
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发表时间：
2012-10-30
期刊：
影响因子：
3.9
通讯作者：
Lequeux, Nicolas
中科院分区：
文献类型：
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作者：
Giovanelli, Emerson;Muro, Eleonora;Lequeux, Nicolas
在水条件下的高胶体稳定性是荧光纳米晶体的先决条件，也被称为“量子点”，打算用于任何长期的生物成像实验。这一基本性质意味着纳米颗粒本身和它们所覆盖的配体之间有很强的亲和力。为了进一步提高我们团队之前开发的双齿单两性离子配体的性能，我们合成了一种多齿多两性离子配体，由双齿单体和单两性离子单体共聚而成。无论介质条件（pH、盐度、稀释度和生物环境）如何，聚合物钝化的纳米晶体都表现出优异的胶体稳定性，并且我们证明了聚合物的亲和力超过了双齿配体的3个数量级（通过竞争实验评估的解吸速率）。多齿多两性离子配体的合成也被证明是容易调节的，并且允许相应量子点的容易功能化，从而导致成功的特异性生物分子靶向。
High colloidal stability in aqueous conditions is a prerequisite for fluorescent nanocrystals, otherwise known as "quantum dots", intended to be used in any long-term bioimaging experiment. This essential property implies a strong affinity between the nanoparticles themselves and the ligands they are coated with. To further improve the properties of the bidentate monozwitterionic ligand previously developed in our team, we synthesized a multidentate polyzwitterionic ligand, issued from the copolymerization of a bidentate monomer and a monozwitterionic one. The nanocrystals passivated by this polymeric ligand showed an exceptional colloidal stability, regardless of the medium conditions (pH, salinity, dilution, and biological environment), and we demonstrated the affinity of the polymer exceeded by 3 orders of magnitude that of the bidentate ligand (desorption rates assessed by a competition experiment). The synthesis of the multidentate polyzwitterionic ligand proved also to be easily tunable and allowed facile functionalization of the corresponding quantum dots, which led to successful specific biomolecules targeting.