Identification of differentially expressed microRNAs during preadipocyte differentiation in Chinese crested duck
Identification of differentially expressed microRNAs during preadipocyte differentiation in Chinese crested duck
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中华凤头鸭前脂肪细胞分化过程中差异表达microRNA的鉴定
DOI：
10.1016/j.gene.2018.03.085
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发表时间：
2018
期刊：
影响因子：
3.5
通讯作者：
Guohong Chen
中科院分区：
文献类型：
--
作者：
Shasha Wang;Yang Zhang;Xiaoya Yuan;Rui Pan;Wencheng Yao;Li Zhong;Qianqian Song;Shenghan Zheng;Zhixiu Wang;Qi Xu;Guobin Chang;Guohong Chen
MicroRNA (miRNA) 被认为是调节广泛生物过程的关键角色。具体而言，据报道 miRNA 在脂肪生成过程中发挥重要作用。在本研究中，我们通过分离脂肪组织来源的前脂肪细胞（WCC）和体外分化72小时后的前脂肪细胞（WCT）构建了脂肪生成模型。对WCT和WCC细胞中表达的miRNA进行深度测序；我们鉴定了 105 个差异表达的 miRNA 候选者（50 个上调，55 个下调）。其中，12 个是新的 miRNA，93 个是以前已知的 miRNA。此外，还选择了 7 个 miRNA 通过逆转录定量 PCR (RT-qPCR) 进行表达确认；结果显示两组间miRNA的差异表达与我们的测序结果一致。其中，miR-223、miR-184-3p和miR-10b-5与脂肪形成有很强的相关性。通过目标预测，我们预测 105 个差异表达的 miRNA 靶向 4155 个独特的 mRNA。对差异表达miRNA靶点的预测表明，miRNA参与多种脂肪生成相关信号通路的调节，包括过氧化物酶体增殖物激活受体（PPAR）信号通路、胰岛素信号通路、脂肪酸生物合成和脂肪酸降解。总的来说，我们的研究结果为进一步研究miRNA提供了背景，并为脂肪生成相关miRNA的预测和分析奠定了基础。
MicroRNAs (miRNAs) are considered key players in the regulation of a broad range of biological processes. Specifically, miRNAs have been reported to play an important role in the process of adipogenesis. In this study, we constructed a model of adipogenesis by isolating preadipocytes (WCC) derived from adipose tissue and preadipocytes after 72 h differentiation (WCT)in vitro. Deep sequencing of miRNAs expressed in WCT and WCC cells was conducted; we identified 105 differentially expressed miRNA candidates (fifty up-regulated and fifty-five down-regulated). Among them, twelve were novel miRNAs, and ninety-three were previously known miRNAs. Furthermore, seven miRNAs were selected for expression confirmation by reverse transcription quantitative PCR (RT-qPCR); the results showed that the differential expression of miRNAs between the two groups was consistent with our sequencing results. Of them, miR-223, miR-184-3p, and miR-10b-5 showed a strong correlation to adipogenesis. Using target prediction, we predicted that the 105 differentially expressed miRNAs targeted 4155 unique mRNAs. The prediction of targets of differentially expressed miRNAs revealed that the miRNAs participated in the regulation of multiple adipogenesis-related signalling pathways, including the peroxisome proliferator-activated receptor (PPAR) signalling pathway, insulin signalling pathway, fatty acid biosynthesis, and fatty acid degradation. Overall, our findings provide a background for further research into miRNAs and lay a foundation for the prediction and analysis of miRNAs related to adipogenesis.