Differential binding of allolactose anomers to the lactose repressor of Escherichia coli.
Differential binding of allolactose anomers to the lactose repressor of Escherichia coli.
复制标题
异乳糖端基异构体与大肠杆菌乳糖阻遏物的差异结合。
DOI：
10.1016/0022-2836(77)90096-1
复制
发表时间：
1977
影响因子：
5.6
通讯作者：
R. E. Huber
中科院分区：
文献类型：
--
作者：
C. L. Turner;R. E. Huber
通过两种方法证明异乳糖的β-端基异构体与大肠杆菌的乳糖阻抑物的优先结合：（1）通过反复洗涤异乳糖阻遏物复合物的硫酸铵沉淀物和（2）通过异丙基-β-d-硫代半乳糖苷竞争性抑制异乳糖结合。定量显示每个异丙基-β-d-硫代半乳糖苷结合位点结合一个β-别乳糖。假设有一个控制系统。
Preferential binding of the β-anomer of allolactose to the lactose repressor ofEscherichia coliwas demonstrated by two methods: (1) by repeated washing of ammonium sulfate precipitates of the allolactose-repressor complex and (2) by competitive inhibition of allolactose binding by isopropyl-β-d-thiogalacto-side. Quantitation showed that one β-allolactose binds per isopropyl-β-d-thiogalactoside binding site. A control system is postulated.