Toxicity of luminescent silica nanoparticles to living cells
Toxicity of luminescent silica nanoparticles to living cells
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DOI：
10.1021/tx7001959
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发表时间：
2007-08-01
影响因子：
4.1
通讯作者：
Zhao, Julia Xiaojun
中科院分区：
文献类型：
--
作者：
Jin, Yuhui;Kannan, Shibichakravarthy;Zhao, Julia Xiaojun
发光纳米材料作为标记材料用于痕量分析物的测定时，可以提供高强度和光稳定的发光信号。然而，出现了一个主要的担忧，即纳米材料是否会对生命系统造成有毒影响。在这里，我们通过系统地研究发光二氧化硅纳米颗粒的细胞毒性和遗传毒性来解决这个问题。这些纳米颗粒是用于生物样品超灵敏测定的强发光标记材料。从两个角度对纳米材料的遗传毒性进行了研究。首先，通过检测DNA碱基修饰、链断裂和DNA修复活性的增加来检测DNA的完整性，以恢复损伤。其次，检测了不同组的细胞DNA，包括核DNA提取物和整个基因组DNA。此外，为了充分评估纳米颗粒对DNA的损伤，分离的基因组DNA直接暴露在纳米颗粒中。通过检测纳米颗粒处理后的细胞增殖率、细胞死亡和死亡模式(坏死和凋亡)来检测纳米颗粒的细胞毒性。结果表明，发光纳米颗粒在分子和细胞水平上没有明显的毒性作用，浓度低于0.1 mg/mL。我们的研究表明，发光二氧化硅纳米颗粒是一种很有前途的生物医学应用标记剂。
Luminescent nanomaterials can provide high-intensity and photostable luminescent signals when used as labeling materials for the determination of trace amounts of analytes. However, a major concern that has arisen is whether the nanomaterials cause toxic effects in living systems. Here, we address this problem through a systematic investigation of the cytotoxicity and genotoxicity of luminescent silica nanoparticles. These nanoparticles are intensely luminescent labeling materials for ultrasensitive determination of biological samples. The investigation of genotoxicity of the nanomaterials was carried out from two perspectives. First, the integrity of the DNA was examined by detecting DNA base modification, strand breaks, and increased DNA repair activity to recover the damage. Second, different sets of cellular DNAs, including nuclear DNA extracts and the whole genomic DNAs, were examined. Furthermore, to fully assess DNA damage by the nanoparticles, isolated genomic DNAs were directly exposed to the nanoparticles. The cytotoxicity of the nanoparticle was detected by measuring the cell proliferation rate, cell death, and death patterns (necrosis and apoptosis) after the nanoparticle treatments. Results show no significant toxic effects due to the luminescent nanoparticles at the molecular and cellular levels below a concentration of 0.1 mg/mL. Our study indicates that the luminescent silica nanoparticle is a promising labeling reagent for various biomedical applications.