Factors affecting full-term development of rat oocytes microinjected with fresh or cryopreserved round spermatids
Factors affecting full-term development of rat oocytes microinjected with fresh or cryopreserved round spermatids
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DOI：
10.1538/expanim.57.401
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发表时间：
2008-07-01
影响因子：
2.4
通讯作者：
Hochi, Shinichi
中科院分区：
文献类型：
--
作者：
Hirabayashi, Masumi;Kato, Megumi;Hochi, Shinichi
以新鲜精子细胞和冷冻精子细胞为研究对象，研究了大鼠卵母细胞圆形精子细胞注射(ROSI)后发育完全的影响因素。无论卵母细胞激活的方法(DC脉冲或离子霉素)和时机(ROSI前后)，注射冷冻精子细胞的卵母细胞发育到完全成熟的比例相似(3.0-4.8%)。在DC处理的卵母细胞中注入新鲜精子细胞(0%)，未获得后代，但先注入新鲜精子细胞，然后用离子霉素激活的卵母细胞发育到完全成熟的比例较高(6.1%)。在进一步的实验中，将离子霉素激活的卵母细胞注射到冷冻解冻的精子细胞中，然后用放线菌素和/或曲古菌素-A处理。两种化学物质对Rosi卵母细胞的发育均无明显影响(3.7-7.9%比2.5%)。综上所述，无论何时激活卵母细胞，注入新鲜精子细胞的大鼠卵母细胞的完全发育都需要离子霉素的激活处理，而当DC脉冲用于卵母细胞激活时，冷冻精子细胞促进完全发育的潜力是显著的。
Factors affecting full-term development of rat oocytes after round spermatid injection (ROSI) were examined using fresh and cryopreserved spermatids. Regardless of method (DC pulses or ionomycin) and timing (before or after ROSI) for oocyte activation, similar percentages of oocytes injected with cryopreserved spermatids developed to full-term (3.0-4.8%). In contrast, no offspring were obtained when fresh spermatids were injected into DC-treated oocytes (0%), but a higher proportion of oocytes first injected with fresh spermatids and then activated with ionomycin developed to full-term (6.1%). In further experiment, oocytes activated with ionomycin were injected with frozen-thawed spermatids, and then treated with cycloheximide and/or trichostatin-A. Neither chemical had a beneficial effect on full-term development of ROSI oocytes (3.7-7.9% vs 2.5% in control). In conclusion, regardless of timing of oocyte activation, activation treatment with ionomycin is required for full-term development of rat oocytes injected with fresh spermatids, and the higher potential of cryopreserved spermatids contributing to full-term development is notable when DC pulse is applied for oocyte activation.