Structural comparison of the yeast cell division cycle gene CDC4 and a related pseudogene.
Structural comparison of the yeast cell division cycle gene CDC4 and a related pseudogene.
复制标题
酵母细胞分裂周期基因 CDC4 和相关假基因的结构比较。
DOI：
10.1016/0022-2836(87)90646-2
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发表时间：
1987
影响因子：
5.6
通讯作者：
Byers,B
中科院分区：
文献类型：
--
作者：
Yochem,J;Byers,B
细胞分裂周期基因CDC 4的功能在酵母中是超越细胞周期G1期所必需的。通过选择互补的隐性温度敏感等位基因从质粒文库中分离野生型基因。基因组序列与克隆基因的杂交揭示了重复序列的存在。对CDC 4和重复序列进行DNA序列分析。这些分析表明：（1）CDC 4含有一个大的开放阅读框，编码779个氨基酸的蛋白质;（2）重复序列与该开放阅读框的羧基端片段具有很强的同源性。在重复序列中存在无义密码子表明它不编码功能产物。破坏酵母基因组中的重复序列为功能提供了更关键的测试。这种破坏没有任何可检测的表型，这证实了该序列应被认为是假基因。插入破坏序列的标记也用于绘制复制图，并确定它与CDC 4没有遗传联系。确定的结构特征表明这些基因之间的进化关系，以及之间的CDC 4产品和其他蛋白质。
The function of the cell division cycle gene,CDC4, is required inSaccharomyces cerevisiaefor progression beyond theG1phase of the cell cycle. The wild-type gene was isolated from a plasmid library by selection for complementation of a recessive, temperature-sensitive allele. Hybridization of genomic sequences with the cloned gene revealed the presence of a duplicated sequence. BothCDC4 and the duplicated sequence were subjected to DNA sequence analysis. These analyses revealed (1) thatCDC4 contains a large open reading frame encoding a protein of 779 amino acids, and (2) that the duplicated sequence bears strong homology with the carboxy-terminal segment of this open reading frame. Presence of a nonsense codon within the duplicated sequence suggested that it does not encode a functional product. Disruption of the duplicated sequence within the yeast genome provided a more critical test for function. The absence of any detectable phenotype for this disruption confirms that the sequence should be considered a pseudogene. The marker inserted to disrupt the sequence also served to map the duplication and to establish that it is not genetically linked toCDC4. The structural features determined suggest evolutionary relationships between these genes as well as between theCDC4 product and other proteins.