Biological and mechanical enhancement of impacted allograft seeded with human bone marrow stromal cells: potential clinical role in impaction bone grafting
Biological and mechanical enhancement of impacted allograft seeded with human bone marrow stromal cells: potential clinical role in impaction bone grafting
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DOI：
10.2217/17460751.1.4.457
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发表时间：
2006-07-01
影响因子：
2.7
通讯作者：
Oreffo, Richard O. C.
中科院分区：
文献类型：
--
作者：
Bolland, Benjamin J. R. F.;Partridge, Kris;Oreffo, Richard O. C.
随着人口老龄化，修复手术的发生率迅速增加。增加骨骼组织损失的临床需要使间充质干细胞与新兴的组织工程学科相结合而脱颖而出。用于髋关节翻修手术的打压植骨是一种公认的骨重建技术，其成功依赖于机械和生物因素的结合。使用颗粒化同种异体移植物是目前公认的临床标准，它提供了良好的机械支架，且具有很少的骨诱导生物潜力。我们建议，应用组织工程范式的原理，将人骨髓基质细胞（hBMSC）与同种异体移植物相结合来产生活的复合材料，与目前单独同种异体移植物的黄金标准相比，具有生物和机械优势。这项研究表明，hBMSC 与同种异体移植物相结合可以承受相当于标准股骨撞击的力，并沿着骨谱系继续分化和增殖。此外，与单独的同种异体移植物相比，活性复合材料具有生物力学优势，具有更高的颗粒间粘合力和剪切强度。
With the demographics of an aging population the incidence of revision surgery is rapidly increasing. Clinical imperatives to augment skeletal tissue loss have brought mesenchymal stem cells to the fore in combination with the emerging discipline of tissue engineering. Impaction bone grafting for revision hip surgery,is a recognized technique to reconstitute bone, the success of which relies on a combination of mechanical and biological factors. The use of morsellized allograft is currently the accepted clinical standard providing a good mechanical scaffold with little osteoinductive biological potential. We propose that applying the principles of a tissue engineering paradigm, the combination of human bone marrow stromal cells (hBMSCs) with allograft to produce a living composite, offers a biological and mechanical advantage over the current gold standard of allograft alone. This study demonstrates that hBMSCs combined with allograft can withstand the forces equivalent to a standard femoral impaction and continue to differentiate and proliferate along the bony lineage. In addition, the living composite provides a biomechanical advantage, with increased interparticulate cohesion and shear strength when compared with allograft alone.