Visualization of interaction between inorganic nanoparticles and bacteria or fungi.
Visualization of interaction between inorganic nanoparticles and bacteria or fungi.
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DOI：
10.2147/ijn.s13532
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发表时间：
2010-12-06
影响因子：
8
通讯作者：
Sokolowska A
中科院分区：
文献类型：
--
作者：
Chwalibog A;Sawosz E;Hotowy A;Szeliga J;Mitura S;Mitura K;Grodzik M;Orlowski P;Sokolowska A
本研究的目的是评估金刚石（纳米d）、银（纳米ag）、金（纳米au）和铂（纳米pt）纳米粒子与金黄色葡萄球菌（细菌）和白色念珠菌（真菌）自组装的形态特征，以确定构建微生物-纳米粒子载体的可能性。将单个纳米颗粒的水胶体添加到金黄色葡萄球菌和白色念珠菌的悬浮液中。混合后立即用透射电子显微镜检查样品。可视化纳米颗粒与微生物之间的形态相互作用表明，纳米d作为电介质，具有正的zeta电位，与微生物的膜电位有很大的不同，并均匀地包裹在微生物周围，而不会对细胞造成明显的损伤和破坏。所有具有负zeta电位的金属纳米粒子都具有细胞损伤特性。纳米银表现出随细胞自组织的特性，能使细胞壁和细胞质膜崩解，并在细胞外释放出一种物质（可能是细胞质）。纳米金与微生物的排列并没有产生自组织系统，而是观察到纳米金与微生物之间的“非接触”相互作用对真菌细胞造成损伤。纳米铂使这两种微生物在细胞外释放一种物质并分解细胞质膜和细胞壁。纳米银、纳米金、纳米铂（都是金属纳米粒子）对细菌和真菌有害。相比之下，纳米d与微生物表面紧密结合，不会对细胞造成明显损伤，并表现出良好的自组装能力。结果表明，这两种微生物都可以作为纳米d的潜在载体。
The objective of the present investigation was to evaluate the morphologic characteristics of self-assemblies of diamond (nano-D), silver (nano-Ag), gold (nano-Au), and platinum (nano-Pt) nanoparticles with Staphylococcus aureus (bacteria) and Candida albicans (fungi), to determine the possibility of constructing microorganism–nanoparticle vehicles. Hydrocolloids of individual nanoparticles were added to suspensions of S. aureus and C. albicans. Immediately after mixing, the samples were inspected by transmission electron microscopy. Visualization of the morphologic interaction between the nanoparticles and microorganisms showed that nano-D, which are dielectrics and exhibit a positive zeta potential, were very different from the membrane potentials of microorganisms, and uniformly surrounded the microorganisms, without causing visible damage and destruction of cells. All metal nanoparticles with negative zeta potential had cell damaging properties. Nano-Ag showed the properties of self-organization with the cells, disintegrating the cell walls and cytoplasmic membranes, and releasing a substance (probably cytoplasm) outside the cell. Arrangement of nano-Au with microorganisms did not create a system of self-organization, but instead a “noncontact” interaction between the nanoparticles and microorganisms was observed to cause damage to fungal cells. Nano-Pt caused both microorganisms to release a substance outside the cell and disintegrated the cytoplasmic membrane and cell wall. Nano-Ag, nano-Au, and nano-Pt (all metal nanoparticles) are harmful to bacteria and fungi. In contrast, nano-D bind closely to the surface of microorganisms without causing visible damage to cells, and demonstrating good self-assembling ability. The results indicate that both microorganisms could be used as potential carriers for nano-D.