The potential role of microRNA‐31 expression in early colorectal cancer
The potential role of microRNA‐31 expression in early colorectal cancer
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DOI：
10.1111/pin.12334
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发表时间：
2015-10
影响因子：
2.2
通讯作者：
Y. Tateishi;K. Okudela;Hideaki Mitsui;S. Umeda;Takehisa Suzuki;Y. Kojima;Kazuteru Watanabe;N. Kawano;I. Endo;K. Ohashi
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文献类型：
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作者：
Y. Tateishi;K. Okudela;Hideaki Mitsui;S. Umeda;Takehisa Suzuki;Y. Kojima;Kazuteru Watanabe;N. Kawano;I. Endo;K. Ohashi
microRNA-31（miR-31）的表达与包括结直肠癌在内的一些人类恶性肿瘤的进展有关。然而，miR-31在粘膜下浸润性（T1）结直肠癌中表达的临床意义仍不清楚。本研究的目的是描述粘膜下浸润性结直肠癌的临床病理特征与致癌调节因子miR-31之间的关系。我们使用真实的定量逆转录-聚合酶链反应（qRT-PCR）研究了50例粘膜下浸润性结直肠癌标本沿着相应的非肿瘤粘膜标本中miR-31的表达。评估miR-31表达水平与临床病理特征之间的关系。使用荧光原位杂交研究miR-31宿主基因座。qRT-PCR显示miR-31在结直肠癌组织中的表达高于非肿瘤组织（P = 0.0002）。miR-31的上调表达可能在结直肠癌的癌变早期发挥致癌作用。
The expression of microRNA‐31 (miR‐31) has been implicated in the progression of some human malignancies including colorectal cancer. However, the clinical significance of the expression of miR‐31 in submucosally invasive (T1) colorectal cancer remains unclear. The aim of the present study was to delineate the relationship between clinicopathological features and the oncogenic modulator miR‐31 in submucosally invasive colorectal cancer. We investigated the expression of miR‐31 in 50 submucosally invasive colorectal cancer specimens, along with the corresponding non‐tumoral mucosa specimens, using a real‐time quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). The relationships between miR‐31 expression levels and clinicopathological characteristics were assessed. The miR‐31 host gene locus was investigated using fluorescence in situ hybridization. qRT‐PCR revealed that the expression of miR‐31 was higher in colorectal cancer tissue than in non‐tumoral tissue (P = 0.0002). The up‐regulated expression of miR‐31 may play an oncogenic role in the early stage of carcinogenesis in colorectal cancers.