The C19 position of 25-hydroxyvitamin D3 faces outward in the vitamin D sterol-binding pocket of vitamin D-binding protein
The C19 position of 25-hydroxyvitamin D3 faces outward in the vitamin D sterol-binding pocket of vitamin D-binding protein
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DOI：
10.1016/s0960-894x(01)00738-7
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发表时间：
2002-02-11
影响因子：
2.7
通讯作者：
Ray, R
中科院分区：
文献类型：
--
作者：
Addo, JK;Swamy, N;Ray, R
放射性标记的25-羟基维生素D-3（25-OH-D-3）的亲和和光亲和类似物与C-19位的探针未能特异性标记维生素D结合蛋白（DBP）的25-OH-D-3结合口袋。然而，在C-3-OH和C-19位置分别具有溴乙酸盐亲和探针和光亲和探针的杂交类似物特异性标记配体结合口袋，表明C-3-OH指向结合腔的“内部”，而C-19位置背向它。
The radiolabeled affinity and photoaffinity analogues of 25-hydroxyvitamin D-3 (25-OH-D-3) with probes at the C-19 position failed to specifically label the 25-OH-D-3-binding pocket of vitamin D-binding protein (DBP). However, a hybrid analogue, with a bromoacetate affinity probe and a photoaffinity probe at C-3-OH and C-19 positions, respectively, specifically labeled the ligand-binding pocket, suggesting that C-3-OH points towards the 'inside' of the binding cavity while the C-19 position faces away from it. (C) 2002 Elsevier Science Ltd. All rights reserved.