NF-κB activation is cell type-specific in the heart.
NF-κB activation is cell type-specific in the heart.
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NF-κB激活在心脏中是细胞类型特异性的。
DOI：
10.1016/j.virol.2016.12.022
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发表时间：
2017-02
期刊：
影响因子：
3.7
通讯作者：
Sherry B
中科院分区：
文献类型：
--
作者：
Rivera-Serrano EE;Sherry B
病毒性心肌炎很常见，可进展为心力衰竭。心肌细胞促炎症反应对于病毒清除至关重要，但持续的炎症反应会导致心脏损伤。转录因子 NF-κB 调节许多促炎细胞因子的表达，但不同心肌细胞类型中 NF-κB 的基础激活和诱导激活尚未进行比较。在这里，我们使用心肌细胞和心脏成纤维细胞的原代培养物来识别心肌细胞类型特异性事件。我们发现，虽然病毒感染很容易刺激心肌成纤维细胞中 NF-κB 的激活，但心肌细胞在很大程度上难以激活 NF-κB。此外，我们发现心肌细胞亚群的 NF-κB 亚细胞定位不同，并将顺式高尔基体识别为心肌细胞特异性宿主区室。总之，结果表明心脏中的 NF-κB 依赖性信号传导是心肌细胞类型特异性的，可能反映了已经进化到平衡反应的机制，这些反应可以保护心脏或损害心脏。
Viral myocarditis is common and can progress to cardiac failure. Cardiac cell pro-inflammatory responses are critical for viral clearance, however sustained inflammatory responses contribute to cardiac damage. The transcription factor NF-κB regulates expression of many proinflammatory cytokines, but basal and induced activation of NF-κB in different cardiac cell types have not been compared. Here, we used primary cultures of cardiac myocytes and cardiac fibroblasts to identify cardiac cell type-specific events. We show that while viral infection readily stimulates activation of NF-κB in cardiac fibroblasts, cardiac myocytes are largely recalcitrant to activation of NF-κB. Moreover, we show that cardiac myocyte subpopulations differ in their NF-κB subcellular localization and identify the cis-Golgi as a cardiac myocyte-specific host compartment. Together, results indicate that NF-κB-dependent signaling in the heart is cardiac cell type-specific, likely reflecting mechanisms that have evolved to balance responses that can be either protective or damaging to the heart.