Nutritional manipulation of primate retinas, III: Effects of lutein or zeaxanthin supplementation on adipose tissue and retina of xanthophyll-free monkeys
Nutritional manipulation of primate retinas, III: Effects of lutein or zeaxanthin supplementation on adipose tissue and retina of xanthophyll-free monkeys
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DOI：
10.1167/iovs.02-1192
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发表时间：
2005-02-01
影响因子：
4.4
通讯作者：
Snodderly, DM
中科院分区：
文献类型：
--
作者：
Johnson, EJ;Neuringer, M;Snodderly, DM
目的。黄斑色素（MP）由叶黄素(L)和玉米黄质(Z)组成，可能有助于预防年龄相关性黄斑变性或延缓其进展。在这项研究中，研究了在没有摄入叶黄素的情况下，补充L或Z对恒河猴血清、脂肪组织和视网膜中类胡萝卜素水平的影响。从出生到7至16岁，18只恒河猴被喂食含有所有必需营养素但不含叶黄素的半纯化饲料。6只添加纯L， 6只添加纯Z，剂量为3.9 μ mol/ kg /天，持续24 ~ 101周。在基线和每隔4至12周，用高效液相色谱法测定脂肪组织中的类胡萝卜素。研究结束时，测定血清和视网膜（中央4毫米、8毫米环和周围）中的类胡萝卜素。结果与喂食标准实验室饮食的对照猴子的数据进行了比较。饲喂无叶黄素饲料的猴子血清和组织中均无L或Z。添加L或Z后，各添加组血清和脂肪组织浓度显著升高。L和3R， 3 ' S- Z （RSZ或meso- Z，不存在于饲料中）都被加入到添加L的猴子的视网膜中，RSZ仅存在于黄斑（中央4mm）。全反式Z在添加Z的猴子视网膜中积累，而非RSZ。L是RSZ的前体，是黄斑色素的主要成分。无叶黄素的猴子可以积累视网膜叶黄素，这为研究它们的吸收和转化提供了一个有价值的模型。
PURPOSE. Macular pigment ( MP) is composed of the xanthophylls lutein ( L) and zeaxanthin ( Z) and may help to prevent age- related macular degeneration or retard its progression. In this study the effects of L or Z supplementation on carotenoid levels was examined in serum, adipose tissue, and retina in rhesus monkeys with no previous intake of xanthophylls.METHODS. From birth to 7 to 16 years of age, 18 rhesus monkeys were fed semipurified diets containing all essential nutrients but no xanthophylls. Six were supplemented with pure L and 6 with pure Z at 3.9 mu mol/ kg per day for 24 to 101 weeks. At baseline and at 4- to 12- week intervals, carotenoids in adipose tissue were measured by HPLC. At study completion, carotenoids in serum and retina ( central 4 mm, 8- mm annulus, and the periphery) were determined. Results were compared with data from control monkeys fed a standard laboratory diet.RESULTS. Monkeys fed xanthophyll- free diets had no L or Z in serum or tissues. After L or Z supplementation, serum and adipose tissue concentrations significantly increased in the supplemented groups. Both L and 3R, 3 ' S- Z ( RSZ or meso- Z, not present in the diet) were incorporated into retinas of monkeys supplemented with L, with RSZ present only in the macula ( central 4 mm). All- trans Z, but no RSZ, accumulated in retinas of monkeys supplemented with Z.CONCLUSIONS. L is the precursor of RSZ, a major component of macular pigment. Xanthophyll- free monkeys can accumulate retinal xanthophylls and provide a valuable model for examining their uptake and conversion.