Vancomycin-resistant enterococci in rooks (Corvus frugilegus) wintering throughout Europe.
Vancomycin-resistant enterococci in rooks (Corvus frugilegus) wintering throughout Europe.
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在整个欧洲越冬的乌鸦（Corvus frugilegus）中存在耐万古霉素肠球菌。
DOI：
10.1111/1462-2920.12002
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发表时间：
2013
影响因子：
5.1
通讯作者：
I. Literák
中科院分区：
文献类型：
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作者：
V. Oravcová;Anuradha Ghosh;L. Zurek;J. Bardón;S. Guenther;A. Čížek;I. Literák
本研究的目的是评估2010/2011年期间在欧洲越冬的白嘴鸦（Corvus frugilegus）中耐万古霉素肠球菌（VRE）的患病率并对其进行表征。选择性培养粪便样品用于VRE并进行表征。脉冲场凝胶电泳和多位点序列分型（MLST）被用来研究流行病学关系的vanA含VRE。在体外测试携带vanA的VRE的万古霉素抗性性状的迁移率。在1073个乌鸦样本中，有62个（6%）发现了VRE。肠球菌物种多样性包括鸡肠球菌（48株），其次是屎肠球菌（9株）和粪肠球菌（5株）。8例VRE携带vanA和ermB基因。7架载有vanA的VRE来自捷克共和国，1架来自德国。所有携带vanA的VRE被鉴定为屎肠球菌。根据MLST分析，6株vanA阳性菌株被归类为ST 92型，1株属于ST 121型，另1株被描述为新类型ST 671。8株分离株中有7株能通过过滤交配传递万古霉素耐药特性，传递率为8.95 ± 3.25 × 10 ~（-7）个接合子/供体。总之，在一些欧洲国家的越冬乌鸦可能会传播临床上重要的肠球菌，并造成环境污染的风险。
This study's aims were to assess the prevalence of, and to characterize, vancomycin-resistant enterococci (VRE) from rooks (Corvus frugilegus) wintering in Europe during 2010/2011. Faeces samples were cultivated selectively for VRE and characterized. Pulsed-field gel electrophoresis and multilocus sequence typing (MLST) were used to examine epidemiologic relationships of vanA-containing VRE. The vanA-carrying VRE were tested in vitro for mobility of vancomycin resistance traits. VRE were found in 62 (6%) of 1073 rook samples. Enterococcal species diversity comprised Enterococcus gallinarum (48 isolates), followed by E. faecium (9) and E. faecalis (5). Eight VRE harboured the vanA and ermB genes. Seven vanA-carrying VRE originated from the Czech Republic and one from Germany. All vanA-carrying VRE were identified as E. faecium. Based on MLST analysis, six vanA-positive isolates were grouped as ST92 type, one isolate belonged to ST121, and the remaining one was described as a novel type ST671. Seven out of eight isolates were able to transfer the vancomycin resistance trait via filter mating with a transfer rate of 8.95 ± 3.25 × 10(-7) transconjugants per donor. In conclusion, wintering rooks in some European countries may disseminate clinically important enterococci and pose a risk for environmental contamination.
Kobayashi K 等人：“胃粘膜相关淋巴组织 (MALT) 低度恶性 B 细胞淋巴瘤生发中心滤泡树突细胞上幽门螺杆菌相关抗原和热休克蛋白 60 的检测”临床 P 杂志
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