THE IMPLICATION OF ADIPOCYTE ATP-BINDING CASSETTE A1 AND G1 TRANSPORTERS IN METABOLIC COMPLICATIONS OF OBESITY
THE IMPLICATION OF ADIPOCYTE ATP-BINDING CASSETTE A1 AND G1 TRANSPORTERS IN METABOLIC COMPLICATIONS OF OBESITY
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DOI：
10.26402/jpp.2019.1.14
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发表时间：
2019-02-01
影响因子：
2.2
通讯作者：
Miklosz, A.
中科院分区：
文献类型：
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作者：
Choromanska, B.;Mysliwiec, P.;Miklosz, A.
肥胖症的特征是脂质代谢失衡，表现为高浓度的循环甘油三酯和总胆固醇以及低的高密度脂蛋白（HDL）水平。与这些脂质相关的异常导致代谢并发症，如2型糖尿病、动脉高血压和心血管疾病。尽管进行了广泛的研究，但仍不清楚为什么一部分肥胖受试者会患上代谢综合征，而另一些则不会。本研究的目的是评估有和无代谢综合征的肥胖受试者内脏和皮下脂肪组织中胆固醇转运蛋白：脂肪细胞ATP结合盒A1（ABCA 1）、脂肪细胞ATP结合盒G1（ABCG 1）、B类清道夫受体（SR-BI）的总表达和质膜表达。为了保持我们的初步研究组的一致性，我们将重点放在女性身上，她们构成了肥胖患者的大多数。该研究在34名患者中进行：24名病态肥胖女性接受减肥手术，其中一半患有代谢综合征; 10名瘦受试者接受择期腹腔镜胆囊切除术。在内脏和皮下脂肪样本中评估胆固醇转运蛋白（SR-BI、ABCA 1和ABCG 1）的总表达和质膜表达，并分析其与其他临床和实验室参数的关系。我们发现，与瘦型患者相比，肥胖型代谢综合征患者内脏脂肪组织中ABCG 1的质膜表达较低。此外，与没有代谢综合征的患者相比，患有代谢综合征的病态肥胖患者的两种类型脂肪组织中的总ABCG 1表达均较低。与瘦型患者相比，无代谢综合征的病态肥胖患者内脏脂肪组织中的质膜ABCA 1表达较低。我们没有发现SR-BI表达的任何显着差异。由于ABCG 1是负责胆固醇流出到HDL，ABCG 1在病态肥胖代谢综合征女性的VAT中的质膜表达减少可能导致血清中HDL浓度显著降低。这也可以通过两个参数之间的高度正相关来证实。
Obesity is characterised by imbalance in lipid metabolism manifested by high concentrations of circulating triacylglycerols and total cholesterol as well as low high-density lipoprotein (HDL) levels. Abnormalities related to these lipids lead to metabolic complications such as type 2 diabetes, arterial hypertension and cardiovascular disease. Despite extensive research, it is still unclear why a subset of obese subjects develop metabolic syndrome, while others do not. The aim of our work was to assess total and plasma membrane expressions of cholesterol transport proteins: adipocyte ATP-binding cassette A1 (ABCA1), adipocyte ATP-binding cassette G1 (ABCG1), class B scavenger receptor (SR-BI) in visceral and subcutaneous adipose tissue of obese subjects with and without metabolic syndrome. To keep our preliminary study group uniform, we focused on women, who constitute the majority of bariatric patients. The study was performed on 34 patients: 24 morbidly obese women subjected to bariatric surgery, half of whom had metabolic syndrome; and 10 lean subjects undergoing elective laparoscopic cholecystectomy. Total and plasma membrane expressions of cholesterol transport proteins (SR-BI, ABCA1 and ABCG1) were assessed in samples of both visceral and subcutaneous adipose and analysed in relation to other clinical and laboratory parameters. We demonstrated lower plasma membrane expressions of ABCG1 in visceral adipose tissue of obese patients with metabolic syndrome as compared to lean ones. In addition, total ABCG1 expressions in both types of adipose tissue were lower in morbidly obese patients with metabolic syndrome compared to those without metabolic syndrome. Plasma membrane ABCA1 expressions in visceral adipose tissue were lower in the group of morbidly obese patients without metabolic syndrome, compared to lean patients. We did not find any significant differences in SR-BI expressions. Because of ABCG1 is responsible for cholesterol efflux to HDL, reduced plasma membrane expression of ABCG1 in VAT of morbidly obese women with metabolic syndrome may leads to a significantly decreased concentration of HDL in serum. This may be also confirmed by high positive correlation between both parameters.