PKA/ATGL signaling pathway is involved in ER stress-mediated lipolysis in adipocytes of grass carp (Ctenopharyngodon idella)
PKA/ATGL signaling pathway is involved in ER stress-mediated lipolysis in adipocytes of grass carp (Ctenopharyngodon idella)
复制标题
PKA/ATGL信号通路参与内质网应激介导的草鱼脂肪细胞脂肪分解
DOI：
10.1007/s10695-021-01032-6
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发表时间：
2022-04-23
影响因子：
2.9
通讯作者：
Ji, Hong
中科院分区：
文献类型：
--
作者：
Ji, Shanghong;Sun, Jian;Ji, Hong
哺乳动物内质网应激（ER应激）与脂肪分解之间的关系已被广泛研究，但在鱼类中相对较少。本研究以草鱼Ctenopharyngodon idella为模型，研究内质网应激对鱼类脂肪细胞脂肪分解的影响。我们发现衣霉素（TM）处理引起的内质网应激显着诱导脂肪细胞的脂肪分解。随后，为了进一步研究蛋白激酶A（PKA）是否参与ER应激诱导的脂肪分解，我们用PKA激活剂forskolin和抑制剂H89处理脂肪细胞。结果表明，其机制与PKA尤其是催化亚基PRKACBa的激活有关。值得注意的是，我们还发现 PKA 通过靶向脂肪甘油三酯脂肪酶 (ATGL) 的 mRNA 水平以及蛋白质和酶活性来调节脂肪分解。综上所述，我们的研究结果表明 PKA/ATGL 信号通路参与 ER 应激介导的草鱼脂肪细胞脂肪分解。为进一步研究鱼类及其他脊椎动物脂肪分解机制提供理论基础。
The relationship between endoplasmic reticulum stress (ER stress) and lipolysis in mammals has been widely studied, but it is relatively scarce in fish. The present study used grass carpCtenopharyngodon idellaas a model to investigate the effect of ER stress on lipolysis in adipocytes of fish. We found that ER stress evoked by tunicamycin (TM) treatment significantly induced lipolysis in adipocytes. Subsequently, in order to further investigate whether protein kinase A (PKA) is involved in ER stress-induced lipolysis, we treated adipocytes with PKA activator forskolin and inhibitor H89. The results showed that the mechanism was related to the activation of PKA, especially the catalytic subunit PRKACBa. Notably, we also found that PKA regulates lipolysis by targeting mRNA level and protein and enzyme activities of adipotriglyceride lipase (ATGL). Taken together, our findings suggest that PKA/ATGL signaling pathway is involved in ER stress-mediated lipolysis of grass carp adipocytes. It provides a theoretical basis for further study on the mechanism of lipolysis in fish and other vertebrates.