Human alpha- and beta-interferon but not gamma- suppress the in vitro replication of LAV, HTLV-III, and ARV-2.
Human alpha- and beta-interferon but not gamma- suppress the in vitro replication of LAV, HTLV-III, and ARV-2.
复制标题
人α-和β-干扰素（但不是γ-）抑制LAV、HTLV-III和ARV-2的体外复制。
DOI：
10.1089/jir.1986.6.143
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Gardner,MB
中科院分区：
文献类型：
--
作者：
Yamamoto,JK;Barre-Sinoussi,F;Bolton,V;Pedersen,NC;Gardner,MB
研究了人干扰素（IFN）（α、β和γ）对艾滋病病毒（LAV、HTLV-III和ARV-2）在人外周血淋巴细胞中体外复制的影响。在病毒产生高峰时，100 U/ml的IFN-α制剂（白细胞、Namalwa、α1和α2）可抑制LAV、HTLV-III和ARV-2复制，通过逆转录酶（RT）活性测定，抑制率大于50%。这种抑制是剂量依赖性的，高剂量（500 U/ml）的IFN-α几乎完全抑制RT活性（77-99%）。低剂量（100 U/ml）的IFN-β抑制所有三种艾滋病病毒的75%。相反，在100 U/ml至500 U/ml的剂量范围内，人IFN-α对感染性病毒的产生没有显著影响。这些结果表明，只有IFN-α和IFN-β对LAV、HTLV-III和ARV-2复制有效。持续供应的IFN似乎是必不可少的RT活性的持续抑制。事实上，单次IFN治疗终止后，病毒产量增加。
The effect of human interferons (IFNs) (alpha, beta, and gamma) on thein vitroreplication of AIDS viruses (LAV, HTLV-III, and ARV-2) in human peripheral blood lymphocytes was investigated. At the time of peak virus production, IFN-α preparations (leukocyte, Namalwa, α1, and α2) at 100 U/ml, suppressed LAV, HTLV-III, and ARV-2 replication as measured by reverse transcriptase (RT) activity by greater than 50%. This suppression was dose dependent and high dosages (500 U/ml) of IFN-α resulted in almost complete suppression of RT activities (77–99%). A low dose (100 U/ml) of IFN-β suppressed all three AIDS viruses by 75%. In contrast, human IFN-α at a dose range from 100 U/ml to 500 U/ml had no significant effect on the production of infectious viruses. These results indicate that only IFN-α and -β are effective against LAV, HTLV-III, and ARV-2 replication. A continuous supply of IFN appeared to be essential for the constant suppression of RT activity. In fact, upon termination of single IFN treatment, enhanced virus production resulted.