Siderophores of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis
Siderophores of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis
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DOI：
10.1016/j.bbrc.2006.07.055
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发表时间：
2006-09-15
影响因子：
3.1
通讯作者：
Byers, B. Rowe
中科院分区：
文献类型：
--
作者：
Wilson, Melissa K.;Abergel, Rebecca J.;Byers, B. Rowe
三种炭疽芽孢杆菌Sterne菌株（USAMRIID、7702和34172）和蜡状芽孢杆菌ATCC 14579分泌两种儿茶酚盐铁载体，petrobactin（其含有3，4-二羟基苯甲酰基部分）和bacillibactin（其含有2，3-二羟基苯甲酰基部分）。然而，杀虫生物苏云金芽孢杆菌ATCC 33679仅产生杆菌素。通过先前分离的铁载体产生的分析[Cendrowski等人，摩尔Microbiol. B.炭疽菌突变株的B.炭疽菌巴卡CEBF操纵子编码杆菌素生产，asbAB基因区是石油杆菌素装配所需的。两个儿茶酚部分也通过单独的路线合成。PCR扩增鉴定了产石油菌素菌株中的asbA和asb B基因，而B基因。苏云金杆菌ATCC 33679仅保留asbA。Petrobactin的合成不限于B的簇。B.蜡样感觉神经组（其中B.蜡状芽孢杆菌（B. cereus）、B.炭疽菌和B.苏云金杆菌被分类），尽管petrobactin可能在对脊椎动物具有致病潜力的菌株中普遍存在。(c)2006年爱思唯尔公司All rights reserved.
Three Bacillus anthracis Sterne strains (USAMRIID, 7702, and 34172) and Bacillus cereus ATCC 14579 excrete two catecholate siderophores, petrobactin (which contains 3,4-dihydroxybenzoyl moieties) and bacillibactin (which contains 2,3-dihydroxybenzoyl moieties). However, the insecticidal organism Bacillus thuringiensis ATCC 33679 makes only bacillibactin. Analyses of siderophore production by previously isolated [Cendrowski et al., Mol. Microbiol. 52 (2004) 407-417] B. anthracis mutant strains revealed that the B. anthracis bacA CEBF operon codes for bacillibactin production and the asbAB gene region is required for petrobactin assembly. The two catecholate moieties also were synthesized by separate routes. PCR amplification identified both asbA and asbB genes in the petrobactin producing strains whereas B. thuringiensis ATCC 33679 retained only asbA. Petrobactin synthesis is not limited to the cluster of B. anthracis strains within the B. cereus sensu lato group (in which B. cereus, B. anthraeis, and B. thuringiensis are classified), although petrobactin might be prevalent in strains with pathogenic potential for vertebrates. (c) 2006 Elsevier Inc. All rights reserved.