Effects of curcumin, D-pinitol alone or in combination in cytotoxicity induced by arsenic in PC12 cells
Effects of curcumin, D-pinitol alone or in combination in cytotoxicity induced by arsenic in PC12 cells
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DOI：
10.1016/j.fct.2020.111577
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发表时间：
2020-10-01
影响因子：
4.3
通讯作者：
Kurasaki, Masaaki
中科院分区：
文献类型：
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作者：
Rahaman, Md Shiblur;Yamasaki, Shojiro;Kurasaki, Masaaki
Arsenic is a well-known potent toxicant affecting people by causing various human diseases. Long-term exposure to arsenic has strong adverse health effects on liver and kidney disorders, and various forms of cancer. Contrarily, curcumin and D-pinitol are bioactive dietary compounds that have antioxidant properties. Both are used to treat a broad variety of human diseases. Thus, we hypothesized that both may have synergistic effects against arsenic-induced toxicity in PC12 cells. Cells were pretreated with curcumin (1, 2.5, 5 and 10 mu M), D-pinitol (1, 2.5, 5 and 10 mu M) alone or in combination, then exposed to sodium arsenite (10 mu M). The final concentration of curcumin 2.5 mu M and D-pinitol 5 mu M was selected for combination treatment based on their highest protection at lowest concentration against arsenic toxicity. Results demonstrated that pretreatment of curcumin and D-pinitol and their combined treatment with arsenic rescued PC12 cells. Western blot analysis results showed that pretreatment of curcumin and D-pinitol and their combined treatment with arsenic significantly inhibited arsenic-induced cell death through up-regulation of pro-survival proteins and down-regulation of cell death-related proteins, although these protein expressions were negatively regulated by arsenic. Furthermore, the effect of combined treatment with curcumin and D-pinitol was stronger than individual treatment.
