Phylogenetic relationships among native Oxytropis species in Turkey using the trnL intron, trnL-F IGS, and trnV intron cpDNA regions
Phylogenetic relationships among native Oxytropis species in Turkey using the trnL intron, trnL-F IGS, and trnV intron cpDNA regions
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DOI：
10.3906/bot-1506-45
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发表时间：
2016-01-01
影响因子：
1.8
通讯作者：
Kaya, Zeki
中科院分区：
文献类型：
--
作者：
Tekpinar, Ayten;Erkul, Seher Karaman;Kaya, Zeki
我们测试了三个叶绿体DNA位点，即trnL（（UAA））内含子，trnL（（UAA））-F-（GAA）基因间间隔区（IGS），和trnV（UAC））内含子，在13个本地棘豆属物种的系统发育效用。我们的目标是确定这些叶绿体DNA标记是否可以是有益的，找出棘豆属物种之间的系统发育关系。为了增加种间取样，从GenBank中检索了9个trnL内含子和trnL-F区域的序列，并将其纳入分析。在数据库中没有trnV内含子区域的序列，因此仅使用天然物种的序列进行分析。最大似然和最大简约性分析的系统发育表明，trnL内含子和trnV内含子区域提供了更好的分辨率的物种之间的关系，相对于trnL-F区域。最高的可变和简约信息位点被观察到在trnL内含子区域，而最低的网站被认为是在trnV内含子。预期trnV内含子区的可变位点较少，因为分析中不包括外源序列。通过trnL内含子和trnL-F区的分析，发现棘豆与本地种在遗传上是分离的，与外来种聚在一起。trnV内含子区的分析结果表明，Oxytropis engizekensis Duman和Vural可能是O.波斯卡以前的一项使用形态特征的研究得出了类似的结论。
We tested the phylogenetic utility of three chloroplast DNA loci, i.e. the trnL((UAA)) intron,trnL((UAA))-F-(GAA) intergenic spacer (IGS), and trnV((UAC)) intron, across thirteen native Oxytropis species. Our objective was to determine whether any of these chloroplast DNA markers could be beneficial to figure out phylogenetic relationships among Oxytropis species. To increase the interspecific sampling, nine sequences of the trnL intron and trnL-F regions were retrieved from GenBank and included in the analyses. No sequence of the trnV intron region was available in the database and so only sequences of the native species were used for the analyses. Phylogenies derived from maximum likelihood and maximum parsimony analyses indicated that the trnL intron and trnV intron regions provided better resolution for relationships among species with respect to the trnL-F region. The highest variable and parsimony informative sites were observed in the trnL intron region, while the lowest sites were seen in the trnV intron. Less variable sites for the trnV intron region were expected since no foreign sequences could be included in the analysis. Oxytropis lazica was phylogenetically separated from native species and clustered with foreign ones when the trnL intron and trnL-F regions were analyzed. The result obtained from the trnV intron region proved that Oxytropis engizekensis Duman and Vural may be a synonym of O. persica Boiss. A previous study that used morphological characters arrived at a similar conclusion.