The effect of cortisol on rabbit fetal lung maturation in vitro.
The effect of cortisol on rabbit fetal lung maturation in vitro.
复制标题
皮质醇对体外兔胎肺成熟的影响。
DOI：
10.1016/0012-1606(81)90242-6
复制
发表时间：
1981
影响因子：
2.7
通讯作者：
Johnston,JM
中科院分区：
文献类型：
--
作者：
Snyder,JM;Mendelson,CR;Johnston,JM
取19天胎兔肺组织，在含或不含胎牛血清的培养液中培养1~11天。根据生化和形态研究的结果，II型肺泡细胞在体外分化的时间与体内成熟的时间相似。在孵化开始时，假定的肺泡上皮细胞未分化，但在9d内，高尔基体和粗面内质网数量增加，腔表面有许多微绒毛和大量板层小体。培养的外植体的管腔内可见分泌的板层小体和管状髓鞘形态。肺组织外植体在含10%胎牛血清的培养液中培养7天后，[~3H]胆碱对磷脂酰胆碱的掺入相对保持不变，但此后增加了一倍。当外植体在含胎牛血清和皮质醇(10−7M)或倍他米松(10−7M)的培养液中维持时，胆碱对磷脂酰胆碱的掺入比在无皮质醇的含血清培养液中维持的高2~3倍。胎肺组织外植体在不含胎牛血清的皮质醇存在下孵育，皮质醇对磷脂酰胆碱的生物合成无刺激作用。因此，血清辅因子是皮质醇促进胎肺发育所必需的。在培养过程中，磷脂酸盐磷酸水解酶(PAPase)的比活力增加到很高的水平。在有血清存在的情况下，皮质醇或倍他米松对磷脂磷酸水解酶的比活力没有影响。
Explants of lung tissue from 19-day gestational age fetal rabbits were maintained in organ culture in medium with or without fetal calf serum for 1 to 11 days. Based on the results of biochemical and morphological studies it was apparent that the type II pneumonocyte differentiatedin vitroat a time similar to that which occurs with maturationin vivo. The epithelial cells of the presumptive alveoli were undifferentiated at the start of incubation, but within 9 days developed increased amounts of Golgi apparatus and rough endoplasmic reticulum, many microvilli on the luminal surface and numerous lamellar bodies. Secreted lamellar bodies and tubular myelin figures were observed in the lumina of cultured explants. The incorporation of [3H]choline into phosphatidylcholine by lung tissue explants maintained in medium containing 10% fetal calf serum remained relatively constant for 7 days of incubation but thereafter increased two-fold. When explants were maintained in fetal calf serum-containing medium and cortisol (10−7M) or betamethasone (10−7M), the incorporation of choline into phosphatidylcholine was two to three times greater than that of explants maintained in serum-containing medium without cortisol. When explants of fetal lung tissue were incubated in the presence of cortisol without fetal calf serum there was no stimulatory effect of cortisol on phosphatidylcholine biosynthesis. Therefore, serum cofactors are necessary for the stimulatory effects of cortisol on fetal lung development. The specific activity of phosphatidate phosphohydrolase (PAPase) increased to very high levels during the culture period. In the presence of serum, cortisol or betamethasone had no effect on the specific activity of phosphatidate phosphohydrolase.