In vivo effects of leptin on autonomic nerve activity and lipolysis in rats
In vivo effects of leptin on autonomic nerve activity and lipolysis in rats
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DOI：
10.1016/j.neulet.2007.02.003
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发表时间：
2007-04-12
影响因子：
2.5
通讯作者：
Nagai, Katsuya
中科院分区：
文献类型：
--
作者：
Shen, Jiao;Tanida, Mamoru;Nagai, Katsuya
瘦素是一种16 kDa的蛋白质，由白色脂肪组织(WAT)产生，被认为是指示脂肪储存大小的反馈信号。大量数据表明，瘦素具有调节脂代谢的作用。然而，其对体内脂质代谢的可能直接影响及其机制尚未完全确定。在本研究中，我们研究了瘦素在体内对自主神经活动和脂解的影响。结果表明，静脉注射瘦素(10 mg/只)可兴奋支配Wat的交感神经，这种作用可被组胺H受体拮抗剂苯海拉明阻断。此外，腹腔注射瘦素(130 mU g/kg)可提高大鼠血浆甘油和游离脂肪酸(FFA)水平。用苯海拉明和P-肾上腺素能受体阻断剂心得安可消除瘦素对血浆FFA的影响，并在视交叉上核(SCN)损毁大鼠消失。我们的结果表明，瘦素可能通过组胺神经元通过H受体驱动交感神经来调节脂肪组织中的脂肪分解过程，而P-肾上腺素能受体，可能是β(3)受体，参与了对瘦素的脂肪分解反应。瘦素在本研究中的作用被认为是由SCN控制的。(C)2007爱思唯尔爱尔兰有限公司。保留所有权利。
Leptin, a 16-kDa protein, is produced by white adipose tissue (WAT), and is thought to serve as a feedback signal indicating the size of fat stores. Considerable amount of data have shown that leptin can mediate lipid metabolism. However, its possible direct effects on the metabolism of lipids in vivo and the mechanisms involved have not been fully characterized. In this study, we investigated the in vivo effects of leptin on the autonomic nerve activity and lipolysis. We found that intravenous administration of leptin (10 mu g/rat) excited the sympathetic nerves innervating WAT, and this effect was abolished by the pretreatment with diphenhydramine, a histamine H, receptor antagonist. Moreover, intraperitoneal administration of leptin (130 mu g/kg) elevated the levels of plasma glycerol and free fatty acid (FFA). The effect of leptin on plasma FFA was eliminated by pretreatment with diphenhydramine and propranolol, a P-adrenergic receptor blocker, and disappeared in suprachiasmatic nucleus (SCN)-lesioned rats. Our results suggest that leptin might regulate the lipolytic processes in adipose tissue through facilitation of the sympathetic nerves, driven by histamine neurons through the H, receptor, and a P-adrenergic receptor, probably the beta(3)-receptor, is involved in the lipolytic response to leptin. The actions of leptin in this study are supposed to be controlled by the SCN. (c) 2007 Elsevier Ireland Ltd. All rights reserved.