Eight genes expression patterns during visual system regeneration in Dugesia japonica
Eight genes expression patterns during visual system regeneration in Dugesia japonica
复制标题
粳稻视觉系统再生过程中八个基因的表达模式
DOI：
10.1016/j.gep.2011.08.001
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发表时间：
2012-01-01
影响因子：
1.2
通讯作者：
Liu, Dezeng
中科院分区：
文献类型：
--
作者：
Dong, Zimei;Yuwen, Yanqing;Liu, Dezeng
三角涡虫因其结构简单、再生能力强等特点，已成为研究视觉系统分子发育过程的理想模式系统。进一步剖析控制D.视觉系统再生的遗传网络的分子事件。japonica的再生眼的形态发生，我们研究了在体视显微镜下的8个基因的转录本的表达水平，参与这一过程的定量实时PCR。这8个基因是Djeya、Djsix-1、Eye 53、DjotxA、Djpax 6、Djopsin、Djnetrin和1020 HH。结果表明，在不同的再生阶段，各基因的表达模式不同，根据不同时间点的表达水平和眼再造过程中的形态发生情况，可将这8个基因分为3组：（1）早期表达组，包括Djeya、Djsix-1、Eye 53和DjotxA，其表达水平在截肢后1 ~ 3d显著升高;（2）中期表达组，仅包含一个基因Djpax 6，其表达水平在第5天达到高峰;（3）晚期表达基因，包括Djopsin、Djnerrin和1020 HH，其转录随着眼再生而逐渐增加。我们的数据表明，眼再造是多基因共同作用的结果，同一组基因在交响乐中可能具有相似的作用或功能。(C)出版社：Elsevier B. V.
Dugesia japonica has become the suitable model system for studying the visual system molecular developmental processes because of their simple structure and high regenerative capacity. To further dissect the molecular events of genetic network controlling the visual system regeneration in D. japonica, we investigated the morphogenesis of regenerating eyes under the stereomicroscope and the transcripts expression levels of eight genes involved in this process by quantitative real-time PCR. The eight genes were Djeya, Djsix-1, Eye53, DjotxA, Djpax6, Djopsin, Djnetrin and 1020HH. The results showed that each gene was of different expression pattern at distinct regeneration stage and these eight genes could be divided into three groups according to the expression levels at different time points and the morphogenesis during eye reconstruction: (1) the early expression group, including Djeya, Djsix-1, Eye53, and DjotxA, which expression levels were significant increase from 1 to 3 days after amputation; (2) the medium-term expression group, only including one gene, Djpax6, which expression level reached the peak on day 5; and (3) the late expression genes, including Djopsin, Djnerrin and 1020HH, which gradually increase transcription with the eye regeneration. Our data suggested that eye reconstruction was the results of polygenic services and the genes in the same group might have similar role or function in symphony. (C) 2011 Published by Elsevier B.V.