Natural allelic variation in a modulator of auxin homeostasis improves grain yield and nitrogen use efficiency in rice
Natural allelic variation in a modulator of auxin homeostasis improves grain yield and nitrogen use efficiency in rice
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DOI：
10.1093/plcell/koaa037
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发表时间：
2021-03-01
期刊：
影响因子：
11.6
通讯作者：
Li, Shan
中科院分区：
文献类型：
--
作者：
Zhang, Siyu;Zhu, Limei;Li, Shan
氮（N）肥料的外部施用是增加作物产量的重要实践。然而，化肥的过度使用显著增加了生产成本并导致环境问题，使得提高作物氮素利用效率（NUE）对未来农业的可持续发展至关重要。在这里，我们表明，水稻（Oryza sativa）NUE数量性状基因座DULL NITROGEN RESPONSE 1（qDNR 1）参与生长素动态平衡，反映了籼稻和粳稻品种之间硝酸盐（NO3-）吸收、氮同化和产量提高的差异。携带DNR 1（indica）等位基因的水稻植株表现出降低的N-响应转录和DNR 1的蛋白质丰度。这反过来又促进生长素的生物合成，从而诱导生长素反应因子介导的NO3-转运蛋白和N-代谢基因的激活，导致提高NUE和谷物产量。我们还表明，在DNR 1基因座的功能丧失突变与增加氮的吸收和同化，导致在中等水平的氮肥投入下提高水稻产量。因此，调节DNR 1介导的生长素反应代表了实现环境可持续的水稻产量提高的一种有前途的策略。
The external application of nitrogen (N) fertilizers is an important practice for increasing crop production. However, the excessive use of fertilizers significantly increases production costs and causes environmental problems, making the improvement of crop N-use efficiency (NUE) crucial for sustainable agriculture in the future. Here we show that the rice (Oryza sativa) NUE quantitative trait locus DULL NITROGEN RESPONSE1 (qDNR1), which is involved in auxin homeostasis, reflects the differences in nitrate (NO3-) uptake, N assimilation, and yield enhancement between indica and japonica rice varieties. Rice plants carrying the DNR1(indica) allele exhibit reduced N-responsive transcription and protein abundance of DNR1. This, in turn, promotes auxin biosynthesis, thereby inducing AUXIN RESPONSE FACTOR-mediated activation of NO3- transporter and N-metabolism genes, resulting in improved NUE and grain yield. We also show that a loss-of-function mutation at the DNR1 locus is associated with increased N uptake and assimilation, resulting in improved rice yield under moderate levels of N fertilizer input. Therefore, modulating the DNR1-mediated auxin response represents a promising strategy for achieving environmentally sustainable improvements in rice yield.