Guidance of glial cell. migration and axonal growth on electrospun nanofibers of poly-ε-caprolactone and a collagen/poly-ε-caprolactone blend
Guidance of glial cell. migration and axonal growth on electrospun nanofibers of poly-ε-caprolactone and a collagen/poly-ε-caprolactone blend
复制标题
DOI：
10.1016/j.biomaterials.2007.03.009
复制
发表时间：
2007-07-01
期刊：
影响因子：
14
通讯作者：
Mey, Joerg
中科院分区：
文献类型：
--
作者：
Schnell, Eva;Klinkhammer, Kristina;Mey, Joerg
我们的长期目标是开发一种人工植入物作为周围神经损伤后轴突再生的管道。在这项研究中，生物可降解的，对齐的聚-ε-己内酯（PCL）和胶原蛋白/PCL（C/PCL）纳米纤维设计为指导结构，通过静电纺丝和细胞培养试验中进行了测试。我们比较了100%PCL的纤维与由25：75%C/PCL共混物组成的纤维。为了测试它们的生物相容性，进行细胞粘附、存活、迁移、对细胞形态的影响、轴突生长和轴突引导的测定。这两种类型的电纺纤维支持定向神经突起生长和神经胶质细胞迁移从背根神经节（DRG）外植体。与纯PCL纤维相比，C/PCL纤维上的雪旺细胞迁移、神经突取向和雪旺细胞、成纤维细胞和嗅鞘细胞的突起形成得到改善。虽然从DRG外植体的轴突伸长的速度在PCL纤维上更高，但分离的感觉神经元的分析显示C/PCL材料的轴突引导显著更好。数据表明，由胶原蛋白和PCL共混物组成的静电纺丝纤维代表了用于支持细胞增殖、过程生长和迁移的合适基质，因此将是用于人工神经植入物的良好材料。(C)2007爱思唯尔有限公司保留所有权利。
Our long-term goal is to develop an artificial implant as a conduit for axonal regeneration after peripheral nerve injury. In this study, biodegradable, aligned poly-epsilon-caprolactone (PCL) and collagen/PCL (C/PCL) nanofibers designed as guidance structures were produced by electrospinning and tested in cell culture assays. We compared fibers of 100% PCL with fibers consisting of a 25:75% C/PCL blend. To test their biocompatibility, assays of cell adhesion, survival, migration, effects on cell morphology, axonal growth and axonal guidance were performed. Both types of eletrospun fibers supported oriented neurite outgrowth and glial migration from dorsal root ganglia (DRG) explants. Schwann cell migration, neurite orientation, and process formation of Schwann cells, fibroblasts and olfactory ensheathing cells were improved on C/PCL fibers, when compared to pure PCL fibers. While the velocity of neurite elongation from DRG explants was higher on PCL fibers, analysis of isolated sensory neurons showed significantly better axonal guidance by the C/PCL material. The data demonstrate that electrospun fibers composed of a collagen and PCL blend represent a suitable substrate for supporting cell proliferation, process outgrowth and migration and as such would be a good material for artificial nerve implants. (C) 2007 Elsevier Ltd. All rights reserved.