A systems biological view of intracellular pathogens.
A systems biological view of intracellular pathogens.
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DOI：
10.1111/j.1600-065x.2010.00998.x
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发表时间：
2011-03
影响因子：
8.7
通讯作者：
Roos DS
中科院分区：
文献类型：
--
作者：
Beiting DP;Roos DS
随着生物医学研究变得越来越数据密集，整合基因组规模的数据集变得越来越重要，以便生成复杂生物过程的更全面的图景。系统生物学范式在策略上可能与传统的还原论科学方法不同，但目标仍然相同：产生站得住脚的假设，推动生物机制的实验阐明。细胞内病原体为系统分析提供了绝佳的机会，因为许多这些生物体都适合基因操作，使其生物学能够与宿主的生物学相抗衡。此外，这些微生物的许多最基本的生物学特性（宿主细胞入侵、免疫逃逸、细胞内复制、长期持续存在）与发病机制直接相关，并且可以使用基因组规模技术轻松量化。在这篇综述中，我们总结并讨论了一些与宿主-病原体相互作用有关的可用和可预见的功能基因组学数据集，并建议宿主-病原体界面代表了系统生物学分析的一个有前途的、易于处理的挑战。成功需要开发和利用新技术，扩大数据采集，并增加公众对综合数据集的访问，以便构建宿主与病原体关系的定量和可测试模型。
As biomedical research becomes increasingly data-intensive, it is increasingly essential to integrate genomic-scale datasets, so as to generate a more holistic picture of complex biological processes. The systems biology paradigm may differ in strategy from traditional reductionist scientific methods, but the goal remains the same: to generate tenable hypotheses driving the experimental elucidation of biological mechanisms. Intracellular pathogens provide an excellent opportunity for systems analysis, as many of these organisms are amenable to genetic manipulation, allowing their biology to be played off against that of the host. Moreover, many of the most fundamental biological properties of these microbes (host cell invasion, immune escape, intracellular replication, long-term persistence) are directly linked to pathogenesis and readily quantifiable using genomic-scale technologies. In this review, we summarize and discuss some of the available and foreseeable functional genomics datasets pertaining to host-pathogen interactions and suggest that the host-pathogen interface represents a promising, tractable challenge for systems biological analysis. Success will require developing and leveraging new technologies, expanding data acquisition, and increasing public access to comprehensive datasets, in order to assemble quantitative and testable models of the host-pathogen relationship.