Thiol-antioxidants interfere with assessing silver nanoparticle cytotoxicity
Thiol-antioxidants interfere with assessing silver nanoparticle cytotoxicity
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DOI：
10.1016/j.nano.2019.102130
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发表时间：
2020-02-01
影响因子：
5.4
通讯作者：
de Jesus, Marcelo B.
中科院分区：
文献类型：
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作者：
Ferreira, Luiz A. B.;dos Reis, Samara Bonesso;de Jesus, Marcelo B.
许多研究表明，银纳米颗粒（AgNP）诱导氧化应激，通常认为这是AgNP细胞毒性的主要机制。大多数这些研究依赖于抗氧化剂来建立这种因果关系;然而，这些抗氧化剂如何与AgNP相互作用的细节往往被忽视。这项工作的目的是研究抗氧化剂与AgNP纳米粒子使用的分子机制。因此，我们研究了巯基抗氧化剂（N-乙酰基-L-半胱氨酸，L-半胱氨酸和谷胱甘肽）或非巯基抗氧化剂（Trolox）与化学和生物合成的AgNP之间的分子相互作用。这两种抗氧化剂都可以减少Huh-7肝癌细胞中活性氧的产生，但只有巯基抗氧化剂可以阻止细胞毒性作用，直接与AgNP结合，导致聚集。我们的研究结果表明，当使用硫醇抗氧化剂来研究金属纳米颗粒与细胞之间的相互作用时，数据解释可能并不简单。这个人工制品说明了可能阻碍纳米技术进步和理解纳米毒性机制的潜在陷阱。(C)2019爱思唯尔公司All rights reserved.
Many studies have shown that silver nanoparticles (AgNP) induce oxidative stress, and it is commonly assumed that this is the main mechanism of AgNP cytotoxicity. Most of these studies rely on antioxidants to establish this cause-and-effect relationship; nevertheless, details on how these antioxidants interact with the AgNP are often overlooked. This work aimed to investigate the molecular mechanisms underlying the use of antioxidants with AgNP nanoparticles. Thus, we studied the molecular interaction between the thiol-antioxidants (N-acetyl-L-Cysteine, L-Cysteine, and glutathione) or non-thiol-antioxidants (Trolox) with chemically and biologically synthesized AgNP. Both antioxidants could mitigate ROS production in Huh-7 hepatocarcinoma cells, but only thiol-antioxidants could prevent the cytotoxic effect, directly binding to the AgNP leading to aggregation. Our findings show that data interpretation might not be straightforward when using thiol-antioxidants to study the interactions between metallic nanoparticles and cells. This artifact exemplifies potential pitfalls that could hinder the progress of nanotechnology and the understanding of the nanotoxicity mechanism. (C) 2019 Elsevier Inc. All rights reserved.