New method for high-performance liquid chromatographic separation and fluorescence detection of ginsenosides
New method for high-performance liquid chromatographic separation and fluorescence detection of ginsenosides
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DOI：
10.1016/s0021-9673(00)01208-5
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发表时间：
2001-03-02
影响因子：
4.1
通讯作者：
Liu, GQ
中科院分区：
文献类型：
--
作者：
Shangguan, D;Han, HW;Liu, GQ
A novel pre-column derivatization method for the quantitative determination of ginsenosides by HPLC with fluorescence detection was established. The double bond at the C-24-C-25 position of ginsenoside was converted into an aldehyde group by means of ozonolysis. Then the aldehyde group reacts with FMOC-hydrazine forming the ginsenoside FMOC-hydrazone. The derivatized products were separated by RP-HPLC with gradient elution. The detection limits of ginsenosides Rg(1) and Rb-1 were 2.0 ng (about 2.5 pmol) and 1.0 ng (about 0.9 pmol), respectively. This method can be used for all ginsenosides having the C-24-C-25 double bond. (C) 2001 Elsevier Science B.V. All rights reserved.
