Respiratory responses of soil micro-organisms to simple and complex organic substrates
Respiratory responses of soil micro-organisms to simple and complex organic substrates
复制标题
土壤微生物对简单和复杂有机基质的呼吸反应
DOI：
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发表时间：
2003
影响因子：
6.5
通讯作者：
D. Hopkins
中科院分区：
文献类型：
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作者：
S. Meli;L. Badalucco;L. English;D. Hopkins
我们已经测量了呼吸速率超过一段时间的13天（304.5小时），在90分钟的时间间隔为两个对比的土壤修改没有，D-葡萄糖，L-丙氨酸，白蛋白，淀粉，D-葡萄糖加L-丙氨酸，D-葡萄糖加白蛋白，L-丙氨酸加淀粉或白蛋白加淀粉在2毫克每克土壤中的每个单独的基板。这些治疗代表了简单和复杂的模型基板添加广泛不同的C：N比，并使调查基板之间的相互作用，因为它们诱导微生物的呼吸反应。当单独加入时，d-葡萄糖发生最大呼吸反应的时间小于15小时，l-丙氨酸约为50小时，白蛋白约为120小时。单独添加淀粉在304.5 h（13天）内未导致呼吸反应。与单独添加的任一基质相比，根据土壤的不同，用L-丙氨酸加D-葡萄糖修正的土壤的峰值呼吸响应提前了6至13小时。从土壤中的呼吸响应模式与d-葡萄糖加白蛋白修正的预测的总和数据相似，这意味着没有相互作用。添加L-丙氨酸加淀粉和白蛋白加淀粉导致呼吸反应增加，这意味着含氮基质促进了土壤微生物对淀粉的利用。
We have measured the rates of respiration over a period of 13 days (304.5 h) at 90-min intervals for two contrasting soils amended with nothing, d-glucose, l-alanine, albumin, starch, d-glucose plus l-alanine, d-glucose plus albumin, l-alanine plus starch or albumin plus starch at 2 mg each individual substrate per gram soil. These treatments represent simple and complex model substrate additions with widely different C:N ratios and enable investigation of the interaction between substrates as they induce microbial respiratory responses. When added singly, the times taken for the maximum respiratory response to occur were less than 15 h for d-glucose, approximately 50 h for l-alanine and approximately 120 h for albumin. Addition of starch alone did not lead to a respiratory response within 304.5 h (13 days). The peak respiratory response from soils amended with l-alanine plus d-glucose was advanced by between 6 and 13 h depending on the soil compared with either substrate added singly. The patterns of respiratory response from soil amended with d-glucose plus albumin were similar to that predicted by the summed data, implying no interaction. The addition of l-alanine plus starch and of albumin plus starch led to increased respiratory responses, implying that the nitrogenous substrate facilitated the utilisation of starch by the soil micro-organisms.