Downregulated Expression of the Cyclase-associated Protein 1 (CAP1) Reduces Migration in Esophageal Squamous Cell Carcinoma
Downregulated Expression of the Cyclase-associated Protein 1 (CAP1) Reduces Migration in Esophageal Squamous Cell Carcinoma
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DOI：
10.1093/jjco/hyt093
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发表时间：
2013-09-01
影响因子：
2.4
通讯作者：
Zhang, Jianguo
中科院分区：
文献类型：
--
作者：
Li, Mei;Yang, Xiaojing;Zhang, Jianguo
环化酶相关蛋白的过表达与几种人类癌症的预后不良有关。环化酶相关蛋白1是促进肿瘤进展的环化酶相关蛋白的成员。为探讨环化酶相关蛋白1（cyclase-associated protein 1，cyclase-associated protein 1）在食管鳞状细胞癌中的表达及其临床病理意义，采用免疫组化和western blot方法检测食管鳞状细胞癌组织中cyclase-associated protein 1的表达。采用KaplanMeier法进行生存分析。通过siRNA敲低cyclase-associated protein 1和过表达cyclase-associated protein 1，研究cyclase-associated protein 1在食管鳞癌细胞系TE 1迁移中的作用。免疫组化结果显示，cyclase-associated protein 1的表达与E-cadherin呈负相关，与淋巴结转移显著相关。生存分析显示，环化酶相关蛋白1过表达与总生存率显著相关（P 0.011）。环化酶相关蛋白1在TE 1细胞中的敲低导致波形蛋白和F-肌动蛋白水平降低以及迁移能力降低。此外，cyclase-associated protein 1的过表达促进了TE 1细胞的迁移，提示cyclase-associated protein 1与食管鳞癌的转移有关，cyclase-associated protein 1的高表达可能提示食管鳞癌患者预后不良。
Overexpression of cyclase-associated proteins has been associated with poor prognosis in several human cancers. Cyclase-associated protein 1 is a member of the cyclase-associated proteins which contributes to tumor progression. The aim of the present study was to examine the expression of cyclase-associated protein 1 and to elucidate its clinicopathologic signicance in a larger series of esophageal squamous cell carcinoma.Immunohistochemical and western blot analyses were performed in esophageal squamous cell carcinoma tissues. Survival analyses were performed by using the KaplanMeier method. The role of cyclase-associated protein 1 in migration was studied in esophageal squamous cell carcinoma cell lines of TE1 through knocking down cyclase-associated protein 1 with siRNA and overexpression of cyclase-associated protein 1. The regulation of cyclase-associated protein 1 on migration was determined by transwell and wound-healing assays.Immunohistochemical analysis showed that cyclase-associated protein 1 expression was negatively associated with E-cadherin and signicantly associated with lymph node metastases. Survival analysis revealed that cyclase-associated protein 1 overexpression was signicantly associated with overall survival (P 0.011). Knock down of cyclase-associated protein 1 in TE1 cells resulted in decreased vimentin and F-actin levels and the capability for migration. In addition, overexpression of cyclase-associated protein 1 promoted the migration of TE1 cells.These ndings suggest that cyclase-associated protein 1 is involved in the metastasis of esophageal squamous cell carcinoma, and that elevated levels of cyclase-associated protein 1 expression may indicate a poor prognosis for patients with esophageal squamous cell carcinoma.