Expression profiles of precursor and mature microRNAs under dehydration and high salinity shock in Populus euphratica
Expression profiles of precursor and mature microRNAs under dehydration and high salinity shock in Populus euphratica
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胡杨脱水和高盐冲击下前体和成熟 microRNA 的表达谱
DOI：
10.1007/s00299-011-1096-9
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发表时间：
2011-10-01
影响因子：
6.2
通讯作者：
Yin, Weilun
中科院分区：
文献类型：
--
作者：
Qin, Yurong;Duan, Zhongxin;Yin, Weilun
MicroRNA (miRNA) 是小型非编码 RNA，通过其靶 mRNA 的裂解或翻译抑制，在植物非生物胁迫反应中发挥重要作用。胡杨是生长在荒漠地区的典型抗逆固着生物。在这里，我们通过胡杨中的 PCR 扩增鉴定了来自 11 个家族的 12 个 miRNA 前体和来自 12 个家族的 13 个成熟 miRNA 的序列。为了检测胡杨中成熟 miRNA 及其前体在脱水和高盐度冲击下的表达差异，我们使用 SYBR Green RT-PCR 检测检测了来自 13 个 miRNA 家族的 14 个 miRNA 前体和来自 17 个 miRNA 家族的 17 个成熟 miRNA。这是胡杨中前体和成熟 miRNA 表达谱的第一份报告。通过分析非生物胁迫休克下的成熟 miRNA 和前体，可以鉴定其加工在胁迫休克环境中受到调节的 miRNA。大多数被预测为植物 miRNA 靶标的基因都涉及发育、抗逆性和代谢过程。我们在体内克隆并通过实验鉴定了 5 个预测靶基因，并同时对同一 RNA 样本中的 5 个靶 mRNA 进行了定量。基于这项研究，我们提出了一些调控途径来说明 miRNA 在响应胡杨非生物胁迫冲击中所发挥的重要作用。
MicroRNAs (miRNAs) are small non-coding RNAs that play vital roles in plant abiotic stress responses via cleavage or translational inhibition of their target mRNAs. Populus euphratica is a typical stress-resistant sessile organism that grows in desert areas. Here, we identified sequences of 12 miRNA precursors from 11 families and 13 mature miRNAs from 12 families by PCR amplification in P. euphratica. To detect expression differences in mature miRNAs and their precursors under dehydration and high salinity shock in P. euphratica, we examined 14 miRNA precursors from 13 miRNA families and 17 mature miRNAs from 17 miRNA families using the SYBR Green RT-PCR assay. This is the first report of expression profiles for both precursor and mature miRNAs in P. euphratica. By profiling both the mature miRNAs and the precursors under abiotic stress shock, it was possible to identify miRNA whose processing is regulated during stress shock environments. A majority of the genes predicted to be targets for plant miRNAs are involved in development, stress resistance and metabolic processes. We have cloned and experimentally identified in vivo five of the predicted target genes and quantified the five target mRNAs from the same RNA sample simultaneously. Based on this study, we propose some regulatory pathways that illustrate the important role that miRNAs play in response to abiotic stress shock in P. euphratica.