An investigation of sudden death in farmed infant Siamese crocodiles during winter and spring in Hainan, China
An investigation of sudden death in farmed infant Siamese crocodiles during winter and spring in Hainan, China
复制标题
海南冬春季养殖暹罗鳄幼崽猝死调查
DOI：
10.18805/ijar.v0iof.6999
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发表时间：
2018-07-01
影响因子：
0.5
通讯作者：
Zheng, Jiping
中科院分区：
文献类型：
--
作者：
Guo, Guiying;Jiang, Jiahao;Zheng, Jiping
2012 - 2013年冬春季节，中国海南省的暹罗鳄养殖场发生了1岁以下幼鳄的猝死事件，但没有任何特殊的临床症状。尸检显示，除腹腔内充满透明腹水外，内脏器官似乎正常。从心脏血液、脾脏和肝脏中共分离到迟缓爱德华氏菌、潘氏变形杆菌、普通变形杆菌、阿氏肠杆菌、弗氏柠檬酸杆菌和藤黄微球菌6种细菌。采用腹腔接种法测定了分离菌株对雌性中国食用蛙（Rana rugulosa）和虎纹蛙（Hoplobatrachus rugulosus）的毒力。六天的死亡率表明E.迟发型血吸虫的致病性最强，而彭氏原杆菌和E. asburiae完全没有表现出感染，其余的表现出类似的不明显的毒性。这些结果表明，E. tarda将是导致暹罗鳄死亡的主要嫌疑人。E.对20种抗菌药物进行了耐药试验。结果表明，头孢他啶、诺氟沙星和阿米卡星对大肠埃希菌高度敏感。迟到。
Sudden deaths without any specific clinical sign occurred among one year old young ones on Siamese crocodile (Crocodylus siamensis) farms in Hainan province, China, during winter and spring in 2012 - 2013. The autopsy showed that the internal organs seemed normal except full of clear ascites in abdominal cavity. There were total six bacterial species isolated, Edwardsiella tarda, Proteus penneri, Proteus vulgaris, Enterobacter asburiae, Citrobacter freundii and Micrococcus luteus from either the heart bloods or the spleens and livers. The virulence of isolated strains was tested by intraperitoneal inoculation in female Chinese edible frogs (Rana rugulosa or Hoplobatrachus rugulosus). The six-day mortality revealed that E. tarda was the most pathogenic, whereas P. penneri and E. asburiae exhibited no infection at all, and the rest demonstrated similar inconspicuous toxicity. All these findings suggest that E. tarda would be the principal suspect causing death of Siamese crocodile. The sensitivities of the E. tarda isolates were tested against 20 kinds of antimicrobial drugs. The results showed that ceftazidime, norfloxacin and amikacin were highly sensitive to the isolates of E. tarda.